Blocking E-selectin inhibits ischaemia-reperfusion-induced neutrophil recruitment to the murine testis

Andrologia
M Celebi, A G A Paul

Abstract

Germ cell-specific apoptosis that occurs after ischaemia-reperfusion (IR) of the murine testis is dependent on neutrophil recruitment to the testis and is dependent upon the cell adhesion molecule E-selectin. In this study, we aimed to inhibit neutrophil recruitment to the IR-induced testis. Mice were subjected to a 2-h period of testicular torsion (ischaemia) followed by detorsion (reperfusion). Shortly after onset of reperfusion, mice received either a function-blocking monoclonal anti-mouse E-selectin antibody (FBmAb) or isotype-matched control antibody. Mice were killed 24 h after reperfusion and cells isolated from the testis were analysed for the presence of neutrophil infiltration by flow cytometry. Administration of FBmAb inhibited neutrophil recruitment to the IR-induced testis dramatically. Therefore, blockage of E-selectin may be a strategy to treat post-ischaemic testis.

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Citations

Mar 20, 2010·Cardiovascular Research·Stephen F Rodrigues, D Neil Granger
Jul 12, 2014·The Journal of Urology·Zoltán BajoryAndrea Szabó
Jun 2, 2017·Experimental and Therapeutic Medicine·Salvatore ArenaCarmelo Romeo

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis