Sep 1, 1977

Blocking of histamine release from human basophils in vitro by the ATPase inhibitor, ethacrynic acid

Clinical and Experimental Immunology
A M Magro

Abstract

Ethacrynic acid, a known inhibitor of both Na+--K+ and Mg2+-activated ATPases, effectively inhibits histamine release from antigen-challenged human basophils in vitro. Ouabain, an inhibitor specific for Na+--K+-activated ATPases, shows no effect upon the quantity of histamine released from the antigen-challenged basophils. Ethacrynic acid also effectively inhibits Ca2+--ionophore A23187-induced release, implying it inhibits the Ca2+-dependent secretory stage of the histamine-release process. Inhibition of ATPases and histamine release by ethacrynic acid both require the presence of the olefinic bond in the ethacrynic-acid molecule. Possible utilization of analogues of ethacrynic acid as anti-allergic drugs and as a device to investigate the ATPase system of histamine-releasing cells is suggested.

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Mentioned in this Paper

Histamine Measurement
Basophils
SpecimenType - Basophils
Adenosine Triphosphatases
A-23187
Ouabain
Sulfhydryl Compounds
Dose-Response Relationship, Immunologic
Immunoglobulin E
Antigens

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