Blocking of iron uptake from transferrin by antibodies against the transferrin binding proteins in Neisseria meningitidis

Microbial Pathogenesis
M PintorC M Ferreirós

Abstract

Neisseria meningitidis, when cultured in iron-restricted environments, synthesises new outer-membrane proteins, many of which are necessary for their survival and growth. Some of these proteins e.g. transferrin-binding proteins 1 and 2 (Tbp1 and Tbp2), are required for the acquisition of iron from transferrin and are examples of important iron-regulated meningococcal surface antigens which are not expressed after growth in common laboratory media. The antigenicity and antigenic heterogeneity of these proteins have been extensively studied, and the bactericidal activity of antibodies directed to them have been studied. In this work we analysed the ability of such antibodies to inhibit transferrin binding and to block iron uptake from human transferrin. Antisera from mice immunized with either meningococcal outer membrane vesicles, purified Tbp1/2 complexes, or purified Tbp2, were incorporated in radiolabeled-iron uptake assays. Uptake was blocked by more than 80% in the homologous strains, but blocked much less efficiently in some heterologous strains, correlating well with inhibition of transferrin binding and with an inhibitory effect on bacterial growth. Inhibition of iron uptake from citrate was unaffected which suggests tha...Continue Reading

Citations

May 26, 1999·Emerging Infectious Diseases·E D Weinberg
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Donna Perkins-BaldingIgor Stojiljkovic
Jan 21, 2000·FEMS Immunology and Medical Microbiology·G TroncosoC M Ferreirós
Feb 13, 2001·Vaccine·A J Pollard, C Frasch
Apr 27, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Seyed Mehdi Larrie BaghalIraj Rasooli

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