PMID: 58932Jul 1, 1976

Blocking of MLC stimulation by anti-Ia sera: studies with the virus plaque assay

The Journal of Immunology : Official Journal of the American Association of Immunologists
S KanoD C Shreffler


The development of congenic mouse strains identical at the H-2K and H-2D loci but differing by I-region associated (Ia) determinants has permitted an association to be established between Ia determinants and stimulation in mixed lymphocyte culture reactions (MLR). The present experiments were undertaken to establish whether the Ir-coded control of MLR operated at the level of recognition or of stimulation. Reciprocal MLR were established between A.TH and A.TL mouse spleen cells in the presence or absence of anti-Ia sera directed either at determinants of the stimulating or responding cells. The number of T cells responding was assessed by the virus plaque assay. Anti-Ia sera directed against the responding cells were no more inhibitory of the MLR than normal mouse serum. In contrast, anti-Ia sera directed against determinants of the mitomycin-treated stimulating cells markedly inhibited activation of T cells in the MLR.

Related Concepts

Antigenic Specificity
Lymphocyte Immune Globulin, Anti-Thymocyte Globulin (Equine)
Cellular Immune Response
Lymphocyte Culture Test, Mixed
Mice, Inbred A
Bacteriophage Plaque Assay

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