Blocking of tissue factor pathway inhibitor (TFPI) shortens the bleeding time in rabbits with antibody induced haemophilia A

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
E ErhardtsenO Nordfang

Abstract

Tissue factor (TF)/FVIIa initiates coagulation by activating factor IX (FIX) and factor X (FX). Tissue factor pathway inhibitor (TFPI)-FXa complexes form and inhibit TF/FVIIa. Blocking of TFPI may facilitate haemostasis initiated by FVIIa/TF thereby compensating for impaired FIX/FVIII-dependent coagulation. This hypothesis was tested in a study using rabbits made temporarily haemophilic by the injection of antibodies against FVIII. These rabbits were given i.v. injections of anti-TFPI IgG antibodies and 40 min later bleeding was initiated by cutting the nail including the apex of the cuticle. Injection of anti-TFPI IgG shortened the bleeding time significantly from 26 min to 11 min (normal mean bleeding time in non-haemophilia rabbits: 5 min). Treatment with anti-TFPI IgG also resulted in a shortening of the haemophilic aPTT to a level slightly longer than the normal aPTT. In addition, the prolonged dilute TF clotting time was shortened by the anti-TFPI IgG treatment. Thus, both bleeding and coagulation parameters indicated that blocking of TFPI may be potentially haemostatic in haemophilia.

Citations

May 22, 2003·Thrombosis Research·Mikael TranholmMorris A Blajchman
Nov 5, 1997·Thrombosis Research·P B OstergaardJ B Hansen
Feb 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Susan A MaroneyAlan E Mast
Sep 12, 2007·Annual Review of Medicine·Ulla Hedner, Mirella Ezban
Apr 12, 2012·Haematologica·Raphaël Marlu, Benoît Polack
Feb 27, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Henrik AgersøIda Hilden
Feb 20, 2002·Haemophilia : the Official Journal of the World Federation of Hemophilia·J F LucíaM A Simón
Mar 13, 2012·Thrombosis Research·Lars C Petersen
Jan 14, 2012·Biologicals : Journal of the International Association of Biological Standardization·Zuben E SaunaBasil Golding
Aug 21, 2010·Thrombosis Research·Manjunath Goolyam BasavarajJohn-Bjarne Hansen
Feb 12, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Susan A Maroney, Alan E Mast
Nov 2, 2005·The Journal of Pathology·Bashir A Lwaleed, Paul S Bass
Jan 16, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·K ØvlisenM Tranholm
May 29, 2012·Journal of Thrombosis and Haemostasis : JTH·M E GorczycaA Srivastava
Nov 13, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·B Tardy-PoncetB Tardy
Nov 3, 2009·Haemophilia : the Official Journal of the World Federation of Hemophilia·F Møller, M Tranholm
Jul 8, 2015·Journal of Thrombosis and Haemostasis : JTH·S A Maroney, A E Mast
May 3, 2014·Thrombosis Research·Cecilia AugustssonLars C Petersen
Mar 13, 2014·Blood·Jeremy P WoodAlan E Mast
May 22, 2016·Thrombosis Research·Julie A PetersonAlan E Mast
Jul 28, 2016·Haemophilia : the Official Journal of the World Federation of Hemophilia·N HakobyanL F Fogg
Dec 1, 2004·The Journal of Laboratory and Clinical Medicine·Ellen BrodinJohn-Bjarne Hansen
Aug 27, 2016·American Journal of Hematology·Jan Hartmann, Stacy E Croteau
Jun 12, 2018·Expert Review of Hematology·Giancarlo Castaman, Silvia Linari
Nov 26, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Alan E Mast
Dec 19, 2018·Blood·Marzia Menegatti, Flora Peyvandi
May 1, 2019·Expert Opinion on Biological Therapy·Giancarlo Castaman, Silvia Linari
Nov 28, 2013·The Journal of Biological Chemistry·Michael DockalFriedrich Scheiflinger

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