Blocking promiscuous activation at cryptic promoters directs cell type-specific gene expression

Science
Jongmin KimMargaret T Fuller

Abstract

To selectively express cell type-specific transcripts during development, it is critical to maintain genes required for other lineages in a silent state. Here, we show in the Drosophila male germline stem cell lineage that a spermatocyte-specific zinc finger protein, Kumgang (Kmg), working with the chromatin remodeler dMi-2 prevents transcription of genes normally expressed only in somatic lineages. By blocking transcription from normally cryptic promoters, Kmg restricts activation by Aly, a component of the testis-meiotic arrest complex, to transcripts for male germ cell differentiation. Our results suggest that as new regions of the genome become open for transcription during terminal differentiation, blocking the action of a promiscuous activator on cryptic promoters is a critical mechanism for specifying precise gene activation.

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Citations

Apr 4, 2018·Epigenetics & Chromatin·Petr P LaktionovStepan N Belyakin
Mar 29, 2019·Development·Susanna E Brantley, Margaret T Fuller
Jun 22, 2019·PLoS Genetics·Benjamin AnguloMargaret T Fuller
Feb 13, 2019·Journal of Medical Genetics·Marilyn Scandaglia, Angel Barco
Nov 9, 2019·Nucleic Acids Research·Igor MačinkovićAlexander Brehm
Jan 31, 2018·Genetics·Ryan J GleasonXin Chen
Jun 3, 2018·Nature Reviews. Molecular Cell Biology·Maria ErmolaevaK Lenhard Rudolph
Jun 28, 2018·Nature Reviews. Molecular Cell Biology·Vanja Haberle, Alexander Stark

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