Blocking SphK1/S1P/S1PR1 Signaling Pathway Alleviates Lung Injury Caused by Sepsis in Acute Ethanol Intoxication Mice.

Inflammation
Liang ChenTangfeng Lv

Abstract

Acute ethanol intoxication increases the risk of sepsis and aggravates the symptoms of sepsis and lung injury. Therefore, this study aimed to explore whether sphingosine kinase 1 (SphK1)/sphingosine-1-phosphate (S1P)/S1P receptor 1 (S1PR1) signaling pathway functions in lung injury caused by acute ethanol intoxication-enhanced sepsis, as well as its underlying mechanism. The acute ethanol intoxication model was simulated by intraperitoneally administering mice with 32% ethanol solution, and cecal ligation and puncture (CLP) was used to construct the sepsis model. The lung tissue damage was observed by hematoxylin-eosin (H&E) staining, and the wet-to-dry (W/D) ratio was used to evaluate the degree of pulmonary edema. Inflammatory cell counting and protein concentration in bronchoalveolar lavage fluid (BALF) were, respectively, detected by hemocytometer and bicinchoninic acid (BCA) method. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and IL-18 in BALF were detected by their commercial enzyme-linked immunosorbent assay (ELISA) kits. The myeloperoxidase (MPO) activity and expression of apoptosis-related proteins and SphK1/S1P/S1PR1 pathway-related proteins were, respectively, analyzed by MPO ELISA kit and...Continue Reading

References

Jun 1, 1995·The Journal of Trauma·V McGillR L Gamelli
Apr 13, 2000·Biochimica Et Biophysica Acta·S Spiegel, S Milstien
Dec 1, 2001·Science·T HlaM J Kluk
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 3, 2003·Nature Reviews. Molecular Cell Biology·Sarah Spiegel, Sheldon Milstien
Oct 8, 2004·Critical Care : the Official Journal of the Critical Care Forum·Vera von DossowClaudia D Spies
Mar 14, 2007·BMC Infectious Diseases·Reetta HuttunenJaana Syrjänen
Jun 24, 2008·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Ellen L BurnhamMarc Moss
Sep 20, 2008·Nature Reviews. Immunology·Daniel RittirschPeter A Ward
Jul 14, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stephen B PruettXiaomin Deng
Mar 27, 2013·Annual Review of Biochemistry·Hugh RosenMichael B A Oldstone
Jun 29, 2013·American Journal of Respiratory and Critical Care Medicine·Ashish J MehtaDavid M Guidot
Jul 3, 2013·Molecular Medicine Reports·Maria LufranoPing Wang
Apr 2, 2015·The Journal of Clinical Investigation·Richard L Proia, Timothy Hla
Nov 22, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Fuyang ZhangLing Tao
Oct 30, 2016·Alcohol·Samantha M YeligarLou Ann S Brown
May 18, 2017·The New England Journal of Medicine·Stephen J Galli
Jun 1, 2017·Journal of Critical Care·Niranjan JeganathanRobert Balk
Nov 12, 2017·Inflammation·Deepika SharmaNarayanan Parameswaran
Apr 4, 2018·The Journal of Thoracic and Cardiovascular Surgery·J Hunter MehaffeyAshish K Sharma
Jun 4, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Felix Nnaemeka Ugwu, Jeffery Ho
May 18, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shenghui ChuZhongbin Deng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis