Blood and lymphatic microvessel damage in irradiated human skin: The role of TGF-β, endoglin and macrophages

Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
Nicola S RussellMarion Scharpfenecker

Abstract

Microvascular damage is an important component of late radiation-induced morbidity. In our pre-clinical models, we demonstrated that repair of vessel injury is dependent on proper endoglin-mediated transforming growth factor-beta (TGF-β) signalling and that it can be affected by infiltrating macrophages. We now wanted to extend these findings in irradiated patients, using skin as a model system, and assess whether bisphosphonates could modulate the response. Paired skin biopsies from irradiated and non-irradiated sites were obtained from 48 breast cancer patients. In 8 patients, biopsies were repeated after 4months of bisphosphonate treatment. Immunohistochemistry was used to assess vascular alterations and leucocyte infiltration. Western Blot and qPCR were used to assess expression of growth factors and their receptors. Decreased blood vessel numbers at early time points were followed by increased endoglin expression and restoration of vessel number. Loss of small lymphatic vessels was associated with increased TGF-β levels, whereas dilation of lymphatic vessels correlated with increased macrophage infiltration. Bisphosphonate treatment reduced leucocyte infiltration, but also prevented restoration of blood vessel numbers afte...Continue Reading

References

Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S L TuckerH D Thames
Dec 1, 1990·International Journal of Radiation Oncology, Biology, Physics·I Turesson
Mar 17, 2000·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·S WachterR Pötter
Sep 3, 2004·The Journal of Clinical Investigation·Enrico GiraudoDouglas Hanahan
Jun 27, 2007·The American Journal of Pathology·Susanne JackowskiPeter Helmbold
May 20, 2009·Annals of Surgical Oncology·Iris M C van der PloegEmiel J T Rutgers
Jul 7, 2009·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Marion ScharpfeneckerFiona A Stewart
Jul 27, 2010·American Journal of Physiology. Heart and Circulatory Physiology·José M López-Novoa, Carmelo Bernabeu
Jun 28, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Marion ScharpfeneckerFiona A Stewart
Nov 25, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Ingar SeemannFiona A Stewart
Jul 16, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Marion ScharpfeneckerFiona A Stewart
May 7, 2014·International Journal of Radiation Biology·Karin de CortieMarion Scharpfenecker
Mar 11, 2015·International Journal of Radiation Oncology, Biology, Physics·Ravi A ChandraAlphonse G Taghian

❮ Previous
Next ❯

Citations

Sep 20, 2015·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·H Peter Rodemann, Stephan Bodis
Apr 12, 2016·Frontiers in Pharmacology·Natacha LeroiPhilippe Martinive
Dec 8, 2017·Radiation Research·Jianyuan SongShuyu Zhang
Aug 15, 2018·Frontiers in Immunology·Inigo Martinez-ZubiaurreTurid Hellevik
Jan 20, 2019·International Journal of Molecular Sciences·Raffaella MarconiLidia Strigari
Oct 2, 2020·Molecular Medicine Reports·Fei XuJingcheng Dong
Mar 7, 2021·International Journal of Molecular Sciences·Wineke BakkerMarie José T H Goumans
Jun 16, 2021·International Journal of Radiation Biology·Marjan BoermaJacqueline P Williams

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

Related Papers

Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
Marion ScharpfeneckerFiona A Stewart
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
Marion ScharpfeneckerFiona A Stewart
American Journal of Respiratory Cell and Molecular Biology
P R Segarini
Advances in Immunology
K MiyazonoC H Heldin
© 2021 Meta ULC. All rights reserved