Blood and Salivary Amphiregulin Levels as Biomarkers for Asthma

Frontiers in Medicine
Mahmood Yaseen HachimRifat Hamoudi

Abstract

Background: Amphiregulin (AREG) expression in asthmatic airways and sputum was shown to increase and correlate with asthma. However, no studies were carried out to evaluate the AREG level in blood and saliva of asthmatic patients. Objective: To measure circulating AREG mRNA and protein concentrations in blood, saliva, and bronchial biopsies samples from asthmatic patients. Methods: Plasma and Saliva AREG protein concentrations were measured using ELISA while PBMCs, and Saliva mRNA expression was measured by RT qPCR in non-severe, and severe asthmatic patients compared to healthy controls. Primary asthmatic bronchial epithelial cells and fibroblasts were assessed for AREG mRNA expression and released soluble AREG in their conditioned media. Tissue expression of AREG was evaluated using immunohistochemistry of bronchial biopsies from asthmatic patients and healthy controls. Publicly available transcriptomic databases were explored for the global transcriptomic profile of bronchial epithelium, and PBMCs were explored for AREG expression in asthmatic vs. healthy controls. Results: Asthmatic patients had higher AREG protein levels in blood and saliva compared to control subjects. Higher mRNA expression in saliva and primary bronchia...Continue Reading

References

Jan 18, 2003·The Journal of Allergy and Clinical Immunology·Stephen T HolgateWilliam R Henderson
Feb 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L M HamiltonD E Davies
Feb 8, 2005·The Journal of Allergy and Clinical Immunology·Shen-Wu WangZhengbin Yao
Jun 16, 2005·The Journal of Investigative Dermatology·Eunkyung ChungRobert J Coffey
Feb 21, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Paola D VermeerJoseph Zabner
Nov 8, 2006·Molecular Medicine·Geneviève PaulissenDidier D Cataldo
Oct 2, 2008·The European Respiratory Journal·P-R Burgel, J A Nadel
Jun 12, 2009·International Archives of Allergy and Immunology·Kenji MatsumotoHirohisa Saito
Sep 9, 2009·Cell Proliferation·S KasinaJ A Macoska
Nov 10, 2009·The Journal of Allergy and Clinical Immunology·Yukinori EnomotoYoshimichi Okayama
Jan 27, 2010·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Gerald Choon-Huat KohDavid Koh
Mar 3, 2010·American Journal of Respiratory and Critical Care Medicine·Silvia NausHermann J Ziltener
Jun 22, 2010·Nature Immunology·Hye Young KimDale T Umetsu
Jul 16, 2010·The Journal of Investigative Dermatology·John T Seykora
Aug 6, 2010·Thorax·Mohib UddinRatko Djukanovic
Oct 6, 2010·Respiratory Research·Andrea ZaniniDario Olivieri
Nov 3, 2010·The Journal of Allergy and Clinical Immunology·Yilin QiTim R Mosmann
Jan 13, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Timothy D Le CrasGurjit K Khurana Hershey
Jun 21, 2011·Immunological Reviews·Nirav R Bhakta, Prescott G Woodruff
Jan 17, 2012·American Journal of Respiratory Cell and Molecular Biology·Masaru UchidaKenji Izuhara
Jan 17, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nobuaki HirotaJames G Martin
Feb 7, 2012·Clinical Biochemistry·Esther A PetersonParaic A Kenny
Mar 20, 2012·Biochemical and Biophysical Research Communications·Shinji FukudaShigeki Higashiyama
Apr 12, 2012·American Journal of Respiratory Cell and Molecular Biology·Nicholas D ManzoBarry R Stripp
May 9, 2012·Nature Medicine·Bart N Lambrecht, Hamida Hammad
Aug 4, 2012·The Journal of Allergy and Clinical Immunology·Guiquan JiaUNKNOWN Bronchoscopic Exploratory Research Study of Biomarkers in Corticosteroid-refractory Asthma (BOBCAT) Study Group
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Mar 15, 2013·The New England Journal of Medicine·Sarah-Jane DawsonNitzan Rosenfeld
Jul 11, 2013·American Journal of Respiratory Cell and Molecular Biology·Jun ChenLinhong Deng
Oct 5, 2013·Clinical Microbiology Reviews·Janice M YoshizawaDavid T W Wong
Oct 24, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K M OreoK J Baines
Oct 29, 2013·Advances in Experimental Medicine and Biology·Regina A Pillai, William J Calhoun
Dec 18, 2013·The European Respiratory Journal·Kian Fan ChungW Gerald Teague
Jan 18, 2014·The European Respiratory Journal·Sabari VallathAdam Giangreco
Jan 24, 2014·Clinical & Developmental Immunology·Yuko MorishimaNobuyuki Hizawa
Jan 28, 2014·Seminars in Cell & Developmental Biology·Carmen Berasain, Matías A Avila
Dec 3, 2014·Molecular Oncology·Christiana S KapplerStephen P Ethier
Mar 6, 2015·NPJ Primary Care Respiratory Medicine·Mike Thomas
Mar 7, 2015·Journal of Leukocyte Biology·Alyssa D GregoryA McGarry Houghton
Jun 7, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Karl Deacon, Alan J Knox
Jul 2, 2015·Current Protocols in Cytometry·Pradeep K Dagur, J Philip McCoy

❮ Previous
Next ❯

Citations

Apr 2, 2021·Frontiers in Cell and Developmental Biology·Mahmood Yaseen HachimRifat Hamoudi
Oct 27, 2021·Journal of Cellular Physiology·Siddharth S SinghRajiv Kumar

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE16032
GSE67472

Methods Mentioned

BETA
blood
Biopsies
ELISA
Biopsy
flow cytometry
nuclear translocation
ubiquitination

Software Mentioned

ImageJ
GraphPad Prism
AREG
GraphPad
GINA
CIBERSORT

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Related Papers

Journal of Gastrointestinal Cancer
Michael J GuzmanHongmiao Sheng
Seminars in Cell & Developmental Biology
Carmen Berasain, Matias A Ávila
Biochimica Et Biophysica Acta
Benoît BusserAmandine Hurbin
International Journal of Molecular Medicine
Yuriko Katoh, Masaru Katoh
© 2021 Meta ULC. All rights reserved