PMID: 6402565Mar 1, 1983Paper

Blood-brain barrier transport of basic amino acids is selectively inhibited at low pH

Journal of Neurochemistry
W OldendorfJ M Diamond

Abstract

The transport of amino acids across the blood-brain barrier was measured with the single-pass carotid injection method. The pH of the injected bolus varied between 4.5 and 8.5. Arginine and lysine uptakes were inhibited 24% at pH 5.5 and 59% at pH 4.5. The uptakes of 2-aminobicyclo (2,2,1) heptane-2-carboxylic acid and phenylalanine were unaffected at this pH. There were also no changes observed in choline, glucose, or butanol transport. The Ki of arginine transport inhibition by H+ was 2.4 +/- 0.5 microM; i.e., pH 5.6 +/- 0.1. No change with pH occurred in the Km of arginine transport, while a significant decrease (p less than 0.01) was observed in the Vmax (10.2 +/- 2.3 nmol min-1 g-1 and 5.6 +/- 2.3 nmol min-1 g-1 at pH 7.5 and pH 5.5, respectively). This noncompetitive inhibition was found to be transient as arginine uptake at pH 7.5; it was measured by carotid injection 30 sec following a previous bolus which was buffered to pH 4.5, and was not significantly different from the control. This selective inhibition of the blood-brain barrier basic amino acid carrier demonstrates the advantage of the carotid injection approach in exposing the capillary exchange site to extreme alterations in chemical composition which could not...Continue Reading

References

Sep 1, 1979·Stroke; a Journal of Cerebral Circulation·W OldendorfE Cornford
Feb 1, 1978·Journal of Neurochemistry·E M CornfordW H Oldendorf
Oct 1, 1978·Endocrinology·E M CornfordW H Oldendorf
Aug 5, 1975·Biochimica Et Biophysica Acta·W M Pardridge, W H Oldendorf
Dec 1, 1975·Journal of Neurochemistry·L A Wade, R Katzman
Jan 1, 1976·The American Journal of Physiology·W H Oldendorf, J Szabo
Feb 27, 1973·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·G BañosO E Pratt
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Dec 1, 1971·The American Journal of Physiology·W H Oldendorf
Sep 1, 1981·Journal of Neurochemistry·L A Wade, H M Brady

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Citations

Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W H OldendorfF L Harris
May 1, 1988·The American Journal of the Medical Sciences·W A Banks, A J Kastin
Nov 1, 1987·Journal of Neurochemistry·Q R SmithS I Rapoport
Apr 1, 1985·Journal of Neurochemistry·W W ShinP C Wong
Dec 31, 2002·Physiological Reviews·Giovanni E MannLuis Sobrevia

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