Blood-brain barrier transport studies, aggregation, and molecular dynamics simulation of multiwalled carbon nanotube functionalized with fluorescein isothiocyanate

International Journal of Nanomedicine
Sergey ShityakovCarola Förster

Abstract

In this study, the ability of a multiwalled carbon nanotube functionalized with fluorescein isothiocyanate (MWCNT-FITC) was assessed as a prospective central nervous system-targeting drug delivery system to permeate the blood-brain barrier. The results indicated that the MWCNT-FITC conjugate is able to penetrate microvascular cerebral endothelial monolayers; its concentrations in the Transwell(®) system were fully equilibrated after 48 hours. Cell viability test, together with phase-contrast and fluorescence microscopies, did not detect any signs of MWCNT-FITC toxicity on the cerebral endothelial cells. These microscopic techniques also revealed presumably the intracellular localization of fluorescent MWCNT-FITCs apart from their massive nonfluorescent accumulation on the cellular surface due to nanotube lipophilic properties. In addition, the 1,000 ps molecular dynamics simulation in vacuo discovered the phenomenon of carbon nanotube aggregation driven by van der Waals forces via MWCNT-FITC rapid dissociation as an intermediate phase.

Citations

Feb 18, 2016·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Bo WangXiaoLing Yan
Sep 4, 2015·Nanomedicine·Julie Tzu-Wen Wang, Khuloud Taysir Al-Jamal
Nov 11, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi-Nan ZhangXiu-Wei Yang
May 21, 2019·Nano Research·Jackson Travis Del Bonis-O'DonnellMarkita P Landry
May 4, 2018·Histochemistry and Cell Biology·Sergey Shityakov, Carola Y Förster
Sep 10, 2019·Journal of Pharmaceutical Sciences· AnnuJaved Ali
Jan 7, 2020·Computer Methods and Programs in Biomedicine·Reza MalekiSara Rostami
Nov 7, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yanli ZhangLongquan Shao

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Methods Mentioned

BETA
fluorescence microscopies
electrophoresis
FCS
fluorescence microscopy

Software Mentioned

MarvinSketch
Lab
Imaris
ImageJ
ACD
Molecular Operating Environment
VMD
MATLAB
GraphPad
GraphPad Prism

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