Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers

Disease Markers
Natália KamodyováPeter Celec

Abstract

Salivary oxidative stress markers represent a promising tool for monitoring of oral diseases. Saliva can often be contaminated by blood, especially in patients with periodontitis. The aim of our study was to examine the impact of blood contamination on the measurement of salivary oxidative stress markers. Saliva samples were collected from 10 healthy volunteers and were artificially contaminated with blood (final concentration 0.001-10%). Next, saliva was collected from 12 gingivitis and 10 control patients before and after dental hygiene treatment. Markers of oxidative stress were measured in all collected saliva samples. Advanced oxidation protein products (AOPP), advanced glycation end products (AGEs), and antioxidant status were changed in 1% blood-contaminated saliva. Salivary AOPP were increased in control and patients after dental treatment (by 45.7% and 34.1%, p < 0.01). Salivary AGEs were decreased in patients after microinjury (by 69.3%, p < 0.001). Salivary antioxidant status markers were decreased in both control and patients after dental treatment (p < 0.05 and p < 0.01). One % blood contamination biased concentrations of salivary oxidative stress markers. Saliva samples with 1% blood contamination are visibly disc...Continue Reading

References

May 1, 1996·Kidney International·V Witko-SarsatB Descamps-Latscha
Jan 21, 2000·Free Radical Biology & Medicine·R L Prior, G Cao
Dec 1, 1963·Acta Odontologica Scandinavica·H LOE, J SILNESS
Feb 26, 2004·Clinical Chemistry·Eve B Schwartz, Douglas A Granger
Aug 4, 2004·Psychoneuroendocrinology·Douglas A GrangerEve B Schwartz
Aug 24, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Natália Kamodyová, Peter Celec
Mar 13, 2013·Disease Markers·Natália KamodyováPeter Celec

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Citations

Oct 17, 2017·International nursing review·J S ChoiL R Donnelly
Dec 5, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Jeong-Hyun Kang, Hong-Seop Kho
Jun 28, 2019·European Journal of Oral Sciences·Alexandra Gaál KovalčíkováĽubomíra Tóthová
Nov 6, 2015·Frontiers in Cellular and Infection Microbiology·L'ubomíra TóthováPeter Celec
Jul 6, 2019·BMC Nephrology·Giancarlo BilancioMassimo Cirillo
Nov 29, 2017·Gerodontology·Jun-Seon ChoiLeeann R Donnelly
Apr 2, 2020·Journal of Clinical Medicine·Pia Lopez-JornetAsta Tvarijonaviciute
Feb 9, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Andrea VernerováFrantišek Švec
May 29, 2021·International Journal of Dental Hygiene·Gloria M RamírezGiuseppe A Romito
May 9, 2018·Journal of Proteome Research·Dominic Burg The U-Biopred Study Group

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Methods Mentioned

BETA
glycation
protein assay
saliva

Software Mentioned

GraphPad
GraphPad Prism
XLStatistics

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