Blood Exposure Causes Ventricular Zone Disruption and Glial Activation In Vitro

Journal of Neuropathology and Experimental Neurology
Leandro Castaneyra-RuizDavid D Limbrick

Abstract

Intraventricular hemorrhage (IVH) is the most common cause of pediatric hydrocephalus in North America but remains poorly understood. Cell junction-mediated ventricular zone (VZ) disruption and astrogliosis are associated with the pathogenesis of congenital, nonhemorrhagic hydrocephalus. Recently, our group demonstrated that VZ disruption is also present in preterm infants with IVH. On the basis of this observation, we hypothesized that blood triggers the loss of VZ cell junction integrity and related cytopathology. In order to test this hypothesis, we developed an in vitro model of IVH by applying syngeneic blood to cultured VZ cells obtained from newborn mice. Following blood treatment, cells were assayed for N-cadherin-dependent adherens junctions, ciliated ependymal cells, and markers of glial activation using immunohistochemistry and immunoblotting. After 24-48 hours of exposure to blood, VZ cell junctions were disrupted as determined by a significant reduction in N-cadherin expression (p < 0.05). This was also associated with significant decrease in multiciliated cells and increase in glial fibrillary acid protein-expressing cells (p < 0.05). These observations suggest that, in vitro, blood triggers VZ cell loss and glial...Continue Reading

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Citations

May 1, 2019·Journal of Neuropathology and Experimental Neurology·Leandro Castañeyra-RuizIbrahim González-Marrero
Feb 18, 2020·Cell and Tissue Research·Roberto HenziMontserrat Guerra
Mar 11, 2020·Nature Reviews. Neurology·Jason K KarimyKristopher T Kahle
Sep 15, 2020·Journal of Neonatal-perinatal Medicine·J J Volpe
Jul 22, 2020·Fluids and Barriers of the CNS·Leandro Castaneyra-RuizDavid D Limbrick
May 6, 2021·Cell Reports·Andrew T HaleEric R Gamazon
Jul 1, 2021·Neuropediatrics·Hans C LudwigSteffi Dreha-Kulaczewski
Jul 16, 2021·Journal of Applied Physiology·Jeff F Dunn, Albert M Isaacs
Jul 30, 2021·Journal of Neuroscience Research·Hans C LudwigHans C Bock

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