Blood-feeding induces reversible functional changes in flight muscle mitochondria of Aedes aegypti mosquito.

PloS One
Renata L S GonçalvesMarcus F Oliveira

Abstract

Hematophagy poses a challenge to blood-feeding organisms since products of blood digestion can exert cellular deleterious effects. Mitochondria perform multiple roles in cell biology acting as the site of aerobic energy-transducing pathways, and also an important source of reactive oxygen species (ROS), modulating redox metabolism. Therefore, regulation of mitochondrial function should be relevant for hematophagous arthropods. Here, we investigated the effects of blood-feeding on flight muscle (FM) mitochondria from the mosquito Aedes aegypti, a vector of dengue and yellow fever. Blood-feeding caused a reversible reduction in mitochondrial oxygen consumption, an event that was parallel to blood digestion. These changes were most intense at 24 h after blood meal (ABM), the peak of blood digestion, when oxygen consumption was inhibited by 68%. Cytochromes c and a+a(3) levels and cytochrome c oxidase activity of the electron transport chain were all reduced at 24 h ABM. Ultrastructural and molecular analyses of FM revealed that mitochondria fuse upon blood meal, a condition related to reduced ROS generation. Consistently, BF induced a reversible decrease in mitochondrial H(2)O(2) formation during blood digestion, reaching their lo...Continue Reading

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Citations

Feb 16, 2011·Insect Biochemistry and Molecular Biology·Jose Henrique M OliveiraCarolina Barillas-Mury
Jul 21, 2012·PloS One·Renata L S GonçalvesCarolina Barillas-Mury
Oct 23, 2016·International Journal for Parasitology. Drugs and Drug Resistance·Danielle Oliveira Dos AnjosMarcos André Vannier-Santos
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Aug 28, 2021·BMC Developmental Biology·Antonio Celestino-MontesFidel de la Cruz Hernández-Hernández

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Datasets Mentioned

BETA
AAT45939

Methods Mentioned

BETA
PCR
transmission electron microscopy

Software Mentioned

GraphPad
GraphPad Prism
Excel
AnaliSYS

Related Concepts