Blood Glucose Determination: Effect of Tube Additives

Advances in Clinical Chemistry
Giuseppe LippiAna-Maria Simundić

Abstract

The measurement of fasting plasma glucose may be biased by a time-dependent decrease of glucose in blood tubes, mainly attributable to blood cell metabolism when glycolysis is not rapidly inhibited or blood cells cannot be rapidly separated from plasma. Although glycolysis inhibitors such as sodium fluoride (NaF) in combination with potassium oxalate (KOx) are currently used for overcoming this drawback, their efficacy for stabilizing blood glucose is seemingly limited, and probably lower than that of newer additives such as the citrate buffer. Therefore, we performed a critical analysis of the current scientific literature aimed to generate evidence-based information about the advantages of using citrate buffer in blood tubes compared to the more conventional NaF additive. The results of our systematic overview of the literature show that citrate blood tubes represent a considerable step forward in achieving more accurate and reliable plasma glucose measurements, thereby limiting the risk of underdiagnosing diabetes due to spurious decrease of glucose concentration in uncentrifuged blood specimens, ensuring higher stability of glucose levels over time, while simultaneously producing less hemolysis compared to NaF blood tubes. ...Continue Reading

Citations

Jun 24, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe LippiAna-Maria Simundic
Nov 24, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe LippiUNKNOWN European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE)
Feb 11, 2020·Clinical Chemistry·David E BrunsDavid B Sacks
Oct 23, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Guillaume GrzychThierry Brousseau
Dec 17, 2019·Biochemia Medica·Isabel García-Del-PinoRubén Gómez-Rioja
Feb 14, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Fiamma BalboniGiuseppe Lippi
Dec 23, 2020·European Journal of Clinical Investigation·Sinem EldemA Seval Ozgu-Erdinc
Oct 28, 2019·Practical Laboratory Medicine·Matthias OrthLieselotte Lennartz
May 1, 2021·Critical Reviews in Clinical Laboratory Sciences·G Lima-OliveiraP Ochoa
Jul 23, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Desirée PerleeGijs den Besten
Aug 28, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Dominika SzokeMauro Panteghini

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