Blood group genotyping by high-throughput DNA analysis: application to the French panel of RBC reagents

Transfusion clinique et biologique : journal de la Société française de transfusion sanguine
Sandrine Kappler-GratiasB-N Pham

Abstract

The use of blood group genotyping for the prediction of antigen expression has been discussed in clinical transfusion settings, but much less for reagent red blood cells selection. In France, the Centre National de Référence pour les Groupes Sanguins (CNRGS) produces a reference panel of reagent red blood cells, mainly used for red cell antibody identification. The use of high-throughput DNA analysis has never been applied to blood donors whose red blood cells are used as reagents. The aim of this study was to compare the serological phenotype and that predicted from DNA analysis in such donors, and to determine the benefit of DNA analysis in reagent red blood cells selection strategy. Red blood cells of 346 blood donors were typed with two different reagents for each antigen. The genotyping was performed by using HEA v1.2 BeadChips, BioArray Solutions, Immucor. The comparison between the serologically determined phenotype and that predicted from DNA analysis held on 8876 paired results obtained from 10 blood group systems and 25 antigens. A 99.95% concordance was observed. Four cases of discrepancy for RH, KEL, LU and DO blood group systems were analyzed. Genotyping precisions were of special interest for the Duffy blood group...Continue Reading

References

Nov 20, 2008·British Journal of Haematology·Neil D Avent
Sep 4, 2009·Human Genetics·Geoff Daniels

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Citations

Jul 31, 2013·British Journal of Haematology·Gregory A Denomme
Aug 20, 2014·British Journal of Haematology·Yann FichouClaude Férec
Oct 2, 2013·Methods : a Companion to Methods in Enzymology·S A BoccozC A Marquette
Apr 12, 2008·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·T PeyrardP Rouger
Nov 23, 2020·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Mohammad Saeed GholamiAkbar Dorgalaleh

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