Blood Group O→A Transformation by Chemical Ligation of Erythrocytes

Chembiochem : a European Journal of Chemical Biology
Ivan M RyzhovNicolai Bovin

Abstract

Agglutination of red blood cells (RBCs) remains the only practical method for routine use for ABH typing in clinical practice. However, exact mechanistic details of agglutination are not yet thoroughly studied. In this research, RBCs of blood group O were converted to blood group A through two approaches: by chemical ligation of the cells' glycocalyx with synthetic blood group A tetrasaccharide, and by insertion of synthetic glycolipid carrying the same A antigen into the cells' membranes. The O→A ligated RBCs and natural A RBCs showed comparable agglutination characteristics with antibodies. As expected, RBCs with inserted glycolipid showed lower agglutination scores. This approach could help cell biologists in site-specific and cell-friendly modification of glycocalyx by other ligands.

References

Oct 29, 2003·Current Opinion in Chemical Biology·Danielle H Dube, Carolyn R Bertozzi
May 4, 2012·Carbohydrate Research·Elena KorchaginaNicolai Bovin
Mar 25, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Maryse St-Louis
Dec 15, 2016·Bioconjugate Chemistry·Xiangang HuangBastien Castagner
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Feb 13, 2018·Scientific Reports·Stephen HenryNicolai Bovin

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Citations

May 22, 2021·Biochimica Et Biophysica Acta. Biomembranes·Eugenia M RapoportNicolai V Bovin

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