Blood meal acquisition enhances arbovirus replication in mosquitoes through activation of the GABAergic system

Nature Communications
Yibin ZhuGong Cheng

Abstract

Mosquitoes are hematophagous insects that carry-on and transmit many human viruses. However, little information is available regarding the common mechanisms underlying the infection of mosquitoes by these viruses. In this study, we reveal that the hematophagous nature of mosquitoes contributes to arboviral infection after a blood meal, which suppresses antiviral innate immunity by activating the GABAergic pathway. dsRNA-mediated interruption of the GABA signaling and blockage of the GABAA receptor by the specific inhibitors both significantly impaired arbovirus replication. Consistently, inoculation of GABA enhanced arboviral infection, indicating that GABA signaling facilitates the arboviral infection of mosquitoes. The ingestion of blood by mosquitoes resulted in robust GABA production from glutamic acid derived from blood protein digestion. The oral introduction of glutamic acid increased virus acquisition by mosquitoes via activation of the GABAergic system. Our study reveals that blood meals enhance arbovirus replication in mosquitoes through activation of the GABAergic system.

References

Dec 1, 1965·The Journal of Physiology·E Van Handel
Jan 1, 1983·Annual Review of Entomology·J L HardyW C Reeves
Jun 3, 1993·Nature·R H Ffrench-ConstantA E Chalmers
Oct 5, 2002·Science·George K ChristophidesFotis C Kafatos
Jul 10, 2004·Insect Biochemistry and Molecular Biology·Fernando G Noriega
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jide TianDaniel L Kaufman
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexander W E FranzKen E Olson
Dec 13, 2006·Nature Biotechnology·Robert E W Hancock, Hans-Georg Sahl
Jun 26, 2007·Science·Robert M WaterhouseGeorge K Christophides
Jun 30, 2007·Journal of Neuroimmunology·Maria Guadalupe Reyes-GarcíaFernando García-Tamayo
Aug 10, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yael Kinel-TahanRon Wides
Feb 6, 2009·Emerging Infectious Diseases·Zhi LuGuo Dong Liang
May 12, 2009·Neurochemistry International·Chandana BuddhalaJang-Yen Wu
Aug 12, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ying WangLingyun Xu
Feb 4, 2010·Pharmacological Reviews·Mikko Uusi-Oukari, Esa R Korpi
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Roopa BhatLawrence Steinman
Nov 26, 2010·Neuropharmacology·Leonardo DionisioMaría Del Carmen Esandi
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nepton SoltaniQinghua Wang
Sep 13, 2011·Journal of Agricultural and Food Chemistry·Maciej A PszczolkowskiJohn J Brown
Dec 14, 2011·Amino Acids·Zhe JinBryndis Birnir
Feb 14, 2013·Journal of the American Mosquito Control Association·Michael J Turell
Mar 22, 2013·The Journal of General Virology·Eili HuhtamoPaolo Ravanini
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Mar 19, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Marc JupinSybren S Wijmenga
Dec 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CarissimoKenneth D Vernick
Jan 7, 2015·Nature Communications·Mathilde GendrinGeorge K Christophides
Feb 19, 2015·Viruses·Carol D Blair, Ken E Olson
Jul 18, 2015·Viruses·Alexander W E FranzRollie J Clem
Oct 23, 2015·PLoS Pathogens·Berlin Londono-RenteriaTonya M Colpitts
Dec 3, 2015·Trends in Parasitology·Gong ChengXiaoping Xiao
Apr 1, 2016·Clinical Microbiology Reviews·Didier Musso, Duane J Gubler
May 14, 2016·Nature Microbiology·Xiaojing PangGong Cheng
May 14, 2016·PLoS Pathogens·Sebastian LequimeLouis Lambrechts

❮ Previous
Next ❯

Citations

Mar 10, 2018·Viruses·William H PalmerRonald P van Rij
Apr 19, 2018·PLoS Neglected Tropical Diseases·Yi-Ping KuoGuann-Yi Yu
Mar 14, 2019·Expert Reviews in Molecular Medicine·Pa WuGong Cheng
Sep 19, 2019·Nature Microbiology·Yibin ZhuGong Cheng
Aug 12, 2018·Insects·Ankit KumarSujatha Sunil
Dec 4, 2019·PLoS Pathogens·Mario NoveloElizabeth A McGraw
May 17, 2019·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Jilei ZhangChengming Wang
Jun 22, 2021·Cellular and Molecular Life Sciences : CMLS·Amol K Bhandage, Antonio Barragan
Apr 20, 2021·Annual Review of Virology·Enhao MaGong Cheng

❮ Previous
Next ❯

Datasets Mentioned

BETA
KU820898.1
GSE91027
GSE91032

Methods Mentioned

BETA
RNA-Seq
PCR
ELISA

Software Mentioned

GraphPad Prism
SOAPaligner

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Comptes rendus hebdomadaires des séances de l'Académie des sciences
P DURANDM HUET
Proceedings of the National Academy of Sciences of the United States of America
Jie XuSara Cherry
Current Opinion in Insect Science
Carol D Blair, Ken E Olson
The Journal of Biological Chemistry
H R CROOKSHANK, B F CLOWDUS
© 2022 Meta ULC. All rights reserved