Blood meal analysis of culicoides (Diptera: ceratopogonidae) in central Tunisia

PloS One
Darine SlamaEmna Chaker

Abstract

To evaluate the host preferences of Culicoides species (Diptera: Ceratopogonidae) in Central Tunisia, we identified the source of blood meals of field collected specimens by sequencing of the cytochrome b (cyt b) mitochondrial locus and Prepronociceptine single copy nuclear gene. The study includes the most common and abundant livestock associated species of biting midges in Tunisia: C. imicola, C. jumineri, C. newsteadi, C. paolae, C. cataneii, C. circumscriptus, C. kingi, C. pseudojumineri, C. submaritimus, C. langeroni, C. jumineri var and some unidentified C. species. Analysis of cyt b PCR products from 182 field collected blood-engorged females' midges revealed that 92% of them fed solely on mammalian species, 1.6% on birds, 2.4% on insects and 0.8% on reptiles. The blast results identified the blood origin of biting midges to the species level with exact or nearly exact matches (≥98%). The results confirm the presence of several Culicoides species, including proven vectors in Central Tunisia. Blood meal analyses show that these species will indeed feed on bigger mammals, thereby highlighting the risk that these viruses will be able to spread in Tunisia.

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Citations

Feb 9, 2016·Medical and Veterinary Entomology·M R VAN DER SaagP D Kirkland
Dec 15, 2015·Frontiers in Veterinary Science·Leila Hadj-HenniDenis Augot
Aug 9, 2016·Parasites & Vectors·Poppy H L LambertonMaría-Gloria Basañez
Jan 10, 2021·Insects·Erin M Borland, Rebekah C Kading

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Datasets Mentioned

BETA
KP337884
KP337885
KP337886
KP337887
KP337888
KP337889
KP337890
KP337891
KP337892
KP337893

Methods Mentioned

BETA
ELISA
PCR
light trap
electrophoresis
dissection

Software Mentioned

Chromas

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