Blood pressure-lowering effects of a Bowman-Birk inhibitor and its derived peptides in normotensive and hypertensive rats.

Scientific Reports
Maria Alzira Garcia de FreitasSonia Maria de Freitas

Abstract

Bioactive plant peptides have received considerable interest as potential antihypertensive agents with potentially fewer side effects than antihypertensive drugs. Here, the blood pressure-lowering effects of the Bowman-Birk protease inhibitor, BTCI, and its derived peptides, PepChy and PepTry, were investigated using normotensive (Wistar-WR) and spontaneously hypertensive rats (SHR). BTCI inhibited the proteases trypsin and chymotrypsin, respectively, at 6 µM and 40 µM, a 10-fold greater inhibition than observed with PepTry (60 µM) and PepChy (400 µM). These molecules also inhibited angiotensin converting enzyme (ACE) with IC50 values of 54.6 ± 2.9; 24.7 ± 1.1; and 24.4 ± 1.1 µM, respectively, occluding its catalytic site, as indicated by molecular docking simulation, mainly for PepChy and PepTry. Gavage administration of BTCI and the peptides promoted a decrease of systolic and diastolic blood pressure and an increase of renal and aortic vascular conductance. These effects were more expressive in SHR than in WR. Additionally, BTCI, PepChy and PepTry promoted coronary vasodilation and negative inotropic effects in isolated perfused hearts. The nitric oxide synthase inhibitor blunted the BTCI and PepChy, with no cardiac effects ...Continue Reading

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Citations

Feb 6, 2021·Analytical Methods : Advancing Methods and Applications·Huifei ZhongRui Zhao
Dec 16, 2020·Protein and Peptide Letters·Tsun-Thai ChaiFai-Chu Wong

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Methods Mentioned

BETA
Reverse-phase chromatography
ion exchange chromatography

Software Mentioned

Open Babel GUI
CHARMM
GraphPad Prism
PepChy
Pymol
PepTry
GRAFIT
LabChart
Origin
ACE

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