Blood transcriptomic markers of necrotizing enterocolitis in preterm pigs.

Pediatric Research
Xiaoyu PanPer T Sangild

Abstract

Necrotizing enterocolitis (NEC), a severe gut disorder in preterm infants, is difficult to predict due to poor specificity and sensitivity of clinical signs and biomarkers. Using preterm piglets as a model, we hypothesized that early development of NEC affects blood gene expression, potentially related to early systemic immune responses. A retrospective analysis of clinical, tissue, and blood data was performed on 129 formula-fed piglets with NEC diagnosis at necropsy on day 5. Subgroups of NEC (n = 20) and control piglets (CON, n = 19) were analyzed for whole-blood transcriptome. Preterm piglets had variable NEC lesions, especially in the colon region, without severe clinical signs (e.g. normal growth, activity, hematology, digestion, few piglets with bloody stools). Transcriptome analysis showed 344 differentially expressed genes (DEGs) between NEC and CON piglets. Validation experiment showed that AOAH, ARG2, FKBP5, PAK2, and STAT3 were among the genes affected by severe lesions on day 5, when analyzed in whole blood and in dried blood spots (DBS). Whole-blood gene expressions may be affected in preterm pigs before clinical signs of NEC get severe. Blood gene expression analysis, potentially using DBS samples, is a novel too...Continue Reading

References

Jan 9, 2004·Archives of Disease in Childhood. Fetal and Neonatal Edition·A Maayan-MetzgerJ Kuint
Apr 9, 2005·Journal of Paediatrics and Child Health·Melissa LuigUNKNOWN NSW & ACT NICUS Group
Jun 14, 2006·The British Journal of Nutrition·Thomas ThymannPer T Sangild
Sep 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cynthia L LeaphartDavid J Hackam
Oct 30, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Fredrika GauffinHåkan Karlsson
Jan 21, 2011·The New England Journal of Medicine·Josef Neu, W Allan Walker
Jun 28, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jayda SiggersMette Boye
Feb 15, 2013·Genetic Testing and Molecular Biomarkers·Dorra BouzidHatem Masmoudi
Jun 25, 2013·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Ann Cathrine F StøyPer T Sangild
Aug 15, 2013·Journal of Animal Science·P T SangildR K Buddington
Nov 29, 2013·BMC Pediatrics·Abdallah OulmaatiJean-Charles Picaud
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 4, 2015·Archives of Disease in Childhood. Fetal and Neonatal Edition·Pak Cheung NgHugh Simon Lam
Jan 22, 2015·The New England Journal of Medicine·Ravi M PatelUNKNOWN Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network
Apr 29, 2015·Pediatric Research·Muqing CaoPer Torp Sangild
Jul 28, 2015·Molecular Genetics and Metabolism·Jonas GrauholmMads V Hollegaard
Dec 23, 2015·The Journal of Clinical Investigation·Charlotte E EganDavid J Hackam
Aug 19, 2016·Nature Reviews. Gastroenterology & Hepatology·Diego F NiñoDavid J Hackam
Jun 11, 2018·Journal of Neuroinflammation·Jing SunStanislava Pankratova
Jul 27, 2018·Italian Journal of Pediatrics·Gabriella D'AngeloEloisa Gitto
Jul 27, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jason L RobinsonJuan C Marini
Aug 18, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shuqiang RenDuc Ninh Nguyen
Jul 10, 2019·PloS One·Sandra Meinich JuhlGorm Greisen
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shuqiang RenDuc Ninh Nguyen
Jun 17, 2020·Frontiers in Immunology·Ole BækPer Torp Sangild
Feb 23, 2021·Frontiers in Pediatrics·Kristine HolgersenPer Torp Sangild
May 20, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shuqiang RenDuc Ninh Nguyen

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Citations

Aug 13, 2021·Frontiers in Immunology·Per T SangildDuc Ninh Nguyen

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