Blood volume and cardiac index in rats after exchange transfusion with hemoglobin-based oxygen carriers

Journal of Applied Physiology
R MigitaR M Winslow

Abstract

We have measured plasma volume and cardiac index in rats after 50% isovolemic exchange transfusion with human hemoglobin cross-linked between the alpha-chains with bis(3,5-dibromosalicyl)fumarate (alpha alpha Hb) and with bovine hemoglobin modified with polyethylene glycol (PEGHb). alpha alpha Hb and PEGHb differ in colloid osmotic pressure (23.4 and 118.0 Torr, respectively), oxygen affinity (oxygen half-saturation pressure of hemoglobin = 30.0 and 10.2 Torr, respectively), viscosity (1.00 and 3.39 cP, respectively), and molecular weight (64,400 and 105,000, respectively). Plasma volume was measured by Evans blue dye dilution modified for interference by plasma hemoglobin. Blood volumes in PEGHb-treated animals were significantly elevated (74.0 +/- 3.5 ml/kg) compared with animals treated with alpha alpha Hb (49.0 +/- 1.2 ml/kg) or Ringer lactate (48.0 +/- 2.0 ml/kg) or with controls (58.2 +/- 1.9 ml/kg). Heart rate reduction after alpha alpha Hb exchange is opposite to that expected with blood volume contraction, suggesting that alpha alpha Hb may have a direct myocardial depressant action. The apparently slow elimination of PEGHb during the 2 h after its injection is a consequence of plasma volume expansion: when absolute he...Continue Reading

References

Dec 1, 1990·Journal of Applied Physiology·B L Brizzee, B R Walker
Jan 1, 1990·Biomaterials, Artificial Cells, and Artificial Organs·C B CliffordC D Smith
Dec 1, 1967·The Journal of Experimental Medicine·S F RabinerL H Friedman
Nov 1, 1993·Journal of Applied Physiology·R Motterlini, V W Macdonald
May 1, 1993·Acta Anaesthesiologica Scandinavica·T TønnessenH Noddeland
Jan 1, 1994·Journal of Applied Physiology·C M GillenE R Nadel
Apr 1, 1993·Journal of Applied Physiology·J R HessW W Brinkley

❮ Previous
Next ❯

Citations

Mar 27, 1999·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·C D ConoverR G Shorr
Jun 14, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A CaronC Vigneron
Aug 31, 2000·Critical Care Medicine·J CreteurJ L Vincent
Mar 29, 2003·Transfusion·Kim D VandegriffRobert M Winslow
Nov 14, 2003·American Journal of Physiology. Endocrinology and Metabolism·Noriko KondoYutaka Oiso
Apr 9, 2005·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·Y SmaniC Vigneron
Dec 15, 2004·Der Anaesthesist·W G VoelckelK H Lindner
Mar 17, 2007·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·T N Estep
Feb 7, 2001·Critical Care Medicine·J Creteur, J L Vincent
Sep 8, 1998·Journal of Applied Physiology·R M WinslowK D Vandegriff
Jun 11, 1999·The American Journal of Physiology·S R FischerG C Kramer
Jan 16, 1999·The Journal of Biological Chemistry·M P DoyleB A Kerwin
Jun 21, 2005·Journal of Cardiovascular Pharmacology·Kakoli Parai, Reza Tabrizchi
Jun 24, 2008·American Journal of Physiology. Heart and Circulatory Physiology·David C IrwinRobert W Gotshall
Apr 14, 2011·Physiological Genomics·Harold G KlemckeKathy L Ryan
May 30, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Timothy N Estep
Jan 16, 2004·Critical Care Medicine·Jacques Creteur, Jean-Louis Vincent
Sep 28, 2000·Journal of Applied Physiology·J CreteurJ L Vincent
Nov 5, 2004·Naunyn-Schmiedeberg's Archives of Pharmacology·Reza Tabrizchi, Carol Ann Ford
Aug 2, 2007·Anesthesiology·Joerg HermannKonrad F Messmer
Jul 15, 2009·São Paulo Medical Journal = Revista Paulista De Medicina·Fabiano Timbó BarbosaLuciano Timbó Barbosa
Jul 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Judith MartiniMarcos Intaglietta
May 11, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Mahendra KavdiaAleksander S Popel
Sep 24, 2004·Transfusion·Paul W Buehler, Abdu I Alayash
Jun 20, 1998·The Journal of Biological Chemistry·R J RohlfsR M Winslow
Apr 23, 2009·Critical Care Medicine·Robert W GotshallDavid C Irwin
Jan 22, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Fantao MengSeetharama A Acharya
Jun 1, 2005·Anesthesiology Clinics of North America·Caroline Thyes, Donat R Spahn
Dec 6, 2005·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·C P Stowell
May 23, 2008·Best Practice & Research. Clinical Anaesthesiology·Roman Kocian, Donat R Spahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.