Bloom's syndrome and PICH helicases cooperate with topoisomerase IIα in centromere disjunction before anaphase.

PloS One
Sébastien RouzeauMounira Amor-Guéret

Abstract

Centromeres are specialized chromosome domains that control chromosome segregation during mitosis, but little is known about the mechanisms underlying the maintenance of their integrity. Centromeric ultrafine anaphase bridges are physiological DNA structures thought to contain unresolved DNA catenations between the centromeres separating during anaphase. BLM and PICH helicases colocalize at these ultrafine anaphase bridges and promote their resolution. As PICH is detectable at centromeres from prometaphase onwards, we hypothesized that BLM might also be located at centromeres and that the two proteins might cooperate to resolve DNA catenations before the onset of anaphase. Using immunofluorescence analyses, we demonstrated the recruitment of BLM to centromeres from G2 phase to mitosis. With a combination of fluorescence in situ hybridization, electron microscopy, RNA interference, chromosome spreads and chromatin immunoprecipitation, we showed that both BLM-deficient and PICH-deficient prometaphase cells displayed changes in centromere structure. These cells also had a higher frequency of centromeric non disjunction in the absence of cohesin, suggesting the persistence of catenations. Both proteins were required for the correct...Continue Reading

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Citations

Nov 22, 2013·Molecular Biology of the Cell·Lina Marcela Gallego-PaezToru Hirota
Dec 18, 2013·Stem Cells and Development·Déborah Afonso CornélioSilvia Regina Batistuzzo de Medeiros
Mar 19, 2014·Breast Cancer Research : BCR·Ivanesa PardoUNKNOWN Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center
Dec 9, 2014·Nature Communications·Nicola BrownlowPeter J Parker
Apr 2, 2013·Cellular and Molecular Life Sciences : CMLS·Kelly A Manthei, James L Keck
Dec 9, 2015·Nature Communications·Christian F NielsenIan D Hickson
Sep 26, 2013·The EMBO Journal·Hocine W MankouriIan D Hickson
Aug 25, 2015·Cell Reports·Takaharu KannoCamilla Sjögren
Apr 15, 2014·Cell Reports·Benoit MiottoPierre-Antoine Defossez
Jul 14, 2016·Nucleic Acids Research·Kevin Z L WuCorrado Santocanale
Oct 21, 2016·Nature Reviews. Molecular Cell Biology·Yves PommierJohn L Nitiss
Feb 7, 2017·Cell Cycle·Michalis Fragkos, Valeria Naim
Jun 10, 2017·Cell Cycle·Jean-Sébastien Hoffmann, Pierre Cordelier
Nov 24, 2017·Cold Spring Harbor Symposia on Quantitative Biology·Kata SarlósIan D Hickson
May 14, 2020·Open Biology·Simon GembleMounira Amor-Guéret
Aug 14, 2020·Genes·Alice FinardiRosella Visintin
Mar 20, 2021·Molecular Genetics and Metabolism·Mouna Ababou

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Datasets Mentioned

BETA
GM08505

Methods Mentioned

BETA
electron microscopy
immunoprecipitation
PCR
ChIP-PCR
FCS
electrophoresis
Assay
transfection
ChIP
reverse

Software Mentioned

ImageJ
Image J
Metamorph

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