PMID: 8961378Jan 1, 1996Paper

Blotting patterns of IgG anti-(U1)RNP antibodies in mixed connective tissue disease

Rheumatology International
A GhirardelloP F Gambari

Abstract

Serum reactivities towards individual U1 snRNP proteins were determined by immunoblotting in 32 patients with mixed connective tissue disease (MCTD). Time persistence of immunoblot profiles and clinical significance of anti-(U1)RNP antibody specificities were also investigated. IgG anti-(U1)RNP antibodies were found in the sera of 29 out of 32 patients (90.6%): 21 (65.6%) reacted with the 70-kD protein, 25 (78.1%) with A, 23 (71.9%) with C and 20 (62.5%) with B/B' proteins. None were reactive with the Sm-D peptide. Seventy kilodalton antibody specificity was strongly associated with a higher antinuclear antibody titre (> 160) and slightly associated with disease activity; anti-B/B' specificity was associated with lymphadenopathy. Anti-A, -C and -B/B' antibodies were negatively associated with systemic lupus erythematosus (SLE) skin rashes. Two types of anti-(U1)RNP blotting patterns were selected: "full spectrum" (53.1% of cases) and a "partially/no reactive" one (46.9%). Such patterns were unchanged over time in 14 out of 16 cases prospectively examined (87.5%), while the pattern shifted from "full spectrum" to "partially/no reactive" in 2 cases (12.5%): in 1 after a prolonged clinical remission (> or = 4 years) and in the oth...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R Lerner, J A Steitz
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1979·Thorax·A M HallJ E Cotes
Feb 13, 1975·The New England Journal of Medicine·A Bohan, J B Peter
Jul 1, 1990·Clinical and Experimental Immunology·U NymanI Pettersson
Oct 1, 1985·European Journal of Immunology·W J HabetsW J Van Venrooij
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P J WhiteS O Hoch
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester

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Citations

Aug 24, 2007·Journal of the European Academy of Dermatology and Venereology : JEADV·W SalmhoferW Graninger
Aug 30, 2011·Circulation Journal : Official Journal of the Japanese Circulation Society·Saori Miyamichi-YamamotoHiroaki Shimokawa
Mar 17, 2019·The Journal of Rheumatology·Birgit Nomeland WitczakIvar Sjaastad

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