BlsA integrates light and temperature signals into iron metabolism through Fur in the human pathogen Acinetobacter baumannii

Scientific Reports
Marisel TuttobeneMaría Alejandra Mussi

Abstract

Light modulates global features of the important human pathogen Acinetobacter baumannii lifestyle including metabolism, tolerance to antibiotics and virulence, most of which depend on the short BLUF-type photoreceptor BlsA. In this work, we show that the ability to circumvent iron deficiency is also modulated by light at moderate temperatures, and disclose the mechanism of signal transduction by showing that BlsA antagonizes the functioning of the ferric uptake regulator (Fur) in a temperature-dependent manner. In fact, we show that BlsA interacts with Fur in the dark at 23 °C, while the interaction is significantly weakened under blue light. Moreover, under iron deprived conditions, expression of Fur-regulated Acinetobactin siderophore genes is only induced in the dark in a BlsA-dependent manner. Finally, growth under iron deficiency is supported in the dark rather than under blue light at moderate temperatures through BlsA. The data is consistent with a model in which BlsA might sequester the repressor from the corresponding operator-promoters, allowing Acinetobactin gene expression. The photoregulation of iron metabolism is lost at higher temperatures such as 30 °C, consistent with fading of the BlsA-Fur interaction at this ...Continue Reading

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Citations

Mar 3, 2019·Applied and Environmental Microbiology·Jeongjoon Choi, Sangryeol Ryu
Jul 11, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alejandro PezzaMaría A Mussi
Jul 10, 2019·Frontiers in Microbiology·Marisel Romina TuttobeneMaría A Mussi
Sep 10, 2019·Frontiers in Microbiology·Adrián E GolicMaría Alejandra Mussi
Jun 30, 2021·Scientific Reports·Marisel Romina TuttobeneMaría Alejandra Mussi

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Methods Mentioned

BETA
two
Two-Hybrid
Y2H
PCR

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