Blu-ray based optomagnetic aptasensor for detection of small molecules

Biosensors & Bioelectronics
Jaeyoung YangMikkel F Hansen

Abstract

This paper describes an aptamer-based optomagnetic biosensor for detection of a small molecule based on target binding-induced inhibition of magnetic nanoparticle (MNP) clustering. For the detection of a target small molecule, two mutually exclusive binding reactions (aptamer-target binding and aptamer-DNA linker hybridization) are designed. An aptamer specific to the target and a DNA linker complementary to a part of the aptamer sequence are immobilized onto separate MNPs. Hybridization of the DNA linker and the aptamer induces formation of MNP clusters. The target-to-aptamer binding on MNPs prior to the addition of linker-functionalized MNPs significantly hinders the hybridization reaction, thus reducing the degree of MNP clustering. The clustering state, which is thus related to the target concentration, is then quantitatively determined by an optomagnetic readout technique that provides the hydrodynamic size distribution of MNPs and their clusters. A commercial Blu-ray optical pickup unit is used for optical signal acquisition, which enables the establishment of a low-cost and miniaturized biosensing platform. Experimental results show that the degree of MNP clustering correlates well with the concentration of a target smal...Continue Reading

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Citations

Jun 1, 2016·Frontiers in Chemistry·Annamaria Ruscito, Maria C DeRosa
Jun 9, 2016·Sensors·Stefan SchrittwieserJoerg Schotter
Jan 17, 2017·Analytical Methods : Advancing Methods and Applications·J YangQ Lin
Jul 7, 2018·ACS Sensors·Edwin En-Te Hwu, Anja Boisen
Jan 21, 2017·Analytical Chemistry·Yan Du, Shaojun Dong

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