Blubber transcriptome responses to repeated ACTH administration in a marine mammal

Scientific Reports
Jared S DeyarminJane I Khudyakov

Abstract

Chronic physiological stress impacts animal fitness by catabolizing metabolic stores and suppressing reproduction. This can be especially deleterious for capital breeding carnivores such as marine mammals, with potential for ecosystem-wide effects. However, the impacts and indicators of chronic stress in animals are currently poorly understood. To identify downstream mediators of repeated stress responses in marine mammals, we administered adrenocorticotropic hormone (ACTH) once daily for four days to free-ranging juvenile northern elephant seals (Mirounga angustirostris) to stimulate endogenous corticosteroid release, and compared blubber tissue transcriptome responses to the first and fourth ACTH administrations. Gene expression profiles were distinct between blubber responses to single and repeated ACTH administration, despite similarities in circulating cortisol profiles. We identified 61 and 12 genes that were differentially expressed (DEGs) in response to the first ACTH and fourth administrations, respectively, 24 DEGs between the first and fourth pre-ACTH samples, and 12 DEGs between ACTH response samples from the first and fourth days. Annotated DEGs were associated with functions in redox and lipid homeostasis, suggest...Continue Reading

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Citations

Aug 8, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jane I KhudyakovDaniel E Crocker
Jun 10, 2020·Integrative and Comparative Biology·Emily K LamJosé Pablo Vázquez-Medina
Sep 10, 2020·Conservation Physiology·Laura Pujade BusquetaJane I Khudyakov
Dec 2, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jared DeyarminJane Khudyakov
Nov 9, 2021·Frontiers in Physiology·Elizabeth R PiotrowskiJane I Khudyakov

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Datasets Mentioned

BETA
SRP157071

Methods Mentioned

BETA
biopsies
biopsy
RNA Assay
RNAseq
PCR
interaction network prediction
glycosylation

Software Mentioned

BLASTX
Extreme Science and Engineering Discovery Environment ( XSEDE )
DAVID Bioinformatics Resources
BLASTP
R
FastQC
Cytoskape
STRING
bcl2Fastq
DIAMOND

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