Blue light induces a neuroprotective gene expression program in Drosophila photoreceptors

BMC Neuroscience
Hana HallVikki M Weake

Abstract

Light exposure induces oxidative stress, which contributes to ocular diseases of aging. Blue light provides a model for light-induced oxidative stress, lipid peroxidation and retinal degeneration in Drosophila melanogaster. In contrast to mature adults, which undergo retinal degeneration when exposed to prolonged blue light, newly-eclosed flies are resistant to blue light-induced retinal degeneration. Here, we sought to characterize the gene expression programs induced by blue light in flies of different ages to identify neuroprotective pathways utilized by photoreceptors to cope with light-induced oxidative stress. To identify gene expression changes induced by blue light exposure, we profiled the nuclear transcriptome of Drosophila photoreceptors from one- and six-day-old flies exposed to blue light and compared these with dark controls. Flies were exposed to 3 h blue light, which increases levels of reactive oxygen species but does not cause retinal degeneration. We identified substantial gene expression changes in response to blue light only in six-day-old flies. In six-day-old flies, blue light induced a neuroprotective gene expression program that included upregulation of stress response pathways and downregulation of gen...Continue Reading

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Citations

Dec 5, 2019·Metallomics : Integrated Biometal Science·Johana Vásquez-ProcopioFanis Missirlis
Apr 3, 2020·International Journal of Molecular Sciences·Nora HeinigCora Roehlecke
Jan 29, 2022·Science Advances·Antonio Torres-MéndezManuel Irimia

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Methods Mentioned

BETA
RNA-seq
environmental stresses
aminoacylation
affinity purifications
RNA seq
PCR

Software Mentioned

mroast
glmTreat
bowtie2
Htseq
HOMER
NIS
Trimmomatic
GOrilla
edgeR
count

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