Blue-White Colony Selection of Virus-Infected Isogenic Recipients Based on a Chrysovirus Isolated from Penicillium italicum

Virologica Sinica
Tingfu ZhangDeli Liu

Abstract

Mycoviruses have been found to infect more than 12 species of Penicillium, but have not been isolated from Penicillium italicum (P. italicum). In this study, we isolated and characterized a new double-stranded RNA (dsRNA) virus, designated Penicillium italicum chrysovirus 1 (PiCV1), from the citrus pathogen P. italicum HSPi-YN1. Viral genome sequencing and molecular characterization indicated that PiCV1 was highly homologous to the previously described Penicillium chrysogenum virus. We further constructed the mutant HSPi-YN1ΔpksP defective in the polyketide synthase gene (pksP), which is involved in pigment biosynthesis, and these mutants formed albino (white) colonies. Then we applied hyphal anastomosis method to horizontally transmit PiCV1 from the white virus-donors (i.e., HSPi-YN1 mutants) to wild-type recipients (i.e., P. italicum strains HSPi-CQ54, HSPi-HB4, and HSPi-HN1), and the desirable PiCV1-infected isogenic recipients, a certain part of blue wild-type strains, can be eventually selected and confirmed by viral genomic dsRNA profile analysis. This blue-white colony screening would be an easier method to select virus-infected P. italicum recipients, according to distinguishable color phenotypes between blue virus-reci...Continue Reading

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Citations

Dec 22, 2020·Frontiers in Microbiology·Aline Midori KanashiroTaícia Pacheco Fill

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Datasets Mentioned

BETA
KU561924.1
KX507075.1
KJ834506.1
AY373910.1
MK214383

Methods Mentioned

BETA
electrophoresis
PCR
RNASample
transmission electron microscopy
density gradient centrifugation
gene
gene knockout

Software Mentioned

DNAMAN
Velvet
MEGA
BLASTN
GENEWIZ
BLASTX
CLUSTAL
SPSS Statistics
BLAST

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