Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

ELife
Ming WangIan T Baldwin

Abstract

High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.

References

Aug 12, 2000·Phytochemistry·W MaierD Strack
Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·F RatcliffD C Baulcombe
Mar 28, 2002·Plant & Cell Physiology·Thomas FesterDieter Strack
Feb 18, 2005·Natural Product Research·Francesca CutilloArmando Zarrelli
Jun 23, 2006·Phytochemistry·Willibald SchliemannDieter Strack
Sep 2, 2006·The New Phytologist·Dieter Strack, Thomas Fester
Apr 4, 2008·Phytochemistry·Willibald SchliemannVictor Wray
Sep 15, 2009·Trends in Plant Science·Deirdre C RooneyAngela Hodge
Oct 23, 2009·The Plant Journal : for Cell and Molecular Biology·Jonathan T VogelHarry J Klee
Mar 2, 2010·Chemical & Pharmaceutical Bulletin·Katsuyoshi MatsunamiYoshio Takeda
Jun 15, 2010·Trends in Plant Science·Ana PinedaMarcel Dicke
Jul 22, 2010·Pharmaceutical Biology·Halisha AishanToru Okuyama
Mar 29, 2011·Chemosphere·David A Vaccari, Nikolay Strigul
Aug 3, 2013·The Plant Journal : for Cell and Molecular Biology·Martin SchäferStefan Meldau
May 1, 1989·Journal of Chemical Ecology·I T Baldwin
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daniela S FlossMaria J Harrison
Jan 1, 1975·Planta·K L Stuart, L B Coke
May 23, 2014·Nature Communications·Rabea SchweigerCaroline Müller
Jul 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Dapeng LiEmmanuel Gaquerel
Jul 24, 2015·Current Opinion in Plant Biology·Rabea Schweiger, Caroline Müller
Aug 27, 2015·The Plant Journal : for Cell and Molecular Biology·Zhihao LingShuqing Xu
Oct 30, 2015·Plant Physiology·Hee-Jin ParkMaria J Harrison
Nov 1, 2015·Biochemical Society Transactions·Lorenzo BorghiEnrico Martinoia
May 22, 2016·Plant Physiology·Walter ChitarraEmilio Guerrieri
Jun 28, 2016·Trends in Plant Science·Xin HouBarry J Pogson
Nov 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dapeng LiEmmanuel Gaquerel
Jun 10, 2017·Science·Leonie H LuginbuehlPeter J Eastmond
Jul 21, 2017·ELife·Andreas KeymerCaroline Gutjahr

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Citations

Oct 16, 2019·Frontiers in Plant Science·Valentina FiorilliSalim Al-Babili
Mar 31, 2019·The Plant Journal : for Cell and Molecular Biology·Rishav RayIan T Baldwin
Dec 2, 2020·The Plant Journal : for Cell and Molecular Biology·Juan C MorenoSalim Al-Babili
Jan 12, 2021·Frontiers in Plant Science·Yu-Heng TsengRalf Oelmüller
Jan 16, 2021·Current Opinion in Plant Biology·Florian Berger, Caroline Gutjahr
Nov 1, 2020·International Journal of Molecular Sciences·Yves Combarnous, Thi Mong Diep Nguyen
Jul 20, 2019·Bio-protocol·Elisabeth MindtIan T Baldwin
Jun 19, 2019·Current Opinion in Plant Biology·Lena M Müller, Maria J Harrison
Nov 19, 2021·Plant Physiology·Anish KunduJyothilakshmi Vadassery

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Datasets Mentioned

BETA
EXX64097.1
as

Methods Mentioned

BETA
glycosylation
transgenic
NMR
RNA-seq
nuclear magnetic resonance
confocal microscopy
PCR

Software Mentioned

R
HyStar
XCMS
STEM Clustering
CAMERA
Bruker TopSpin

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