BM61 of Bombyx mori nucleopolyhedrovirus: its involvement in the egress of nucleocapsids from the nucleus

FEBS Letters
Hongxing Shen, Keping Chen

Abstract

All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm.

References

Jun 4, 1999·The Journal of General Virology·S GomiS Maeda
Nov 5, 2002·Annual Review of Entomology·Elisabeth A HerniouDavid R O'Reilly
Sep 22, 2005·Virus Genes·Yi LiXunzhang Wang
Nov 22, 2005·The Journal of General Virology·Vivienne L YoungVernon K Ward
Nov 11, 2006·Virus Genes·Qianyun XiXunzhang Wang
Mar 26, 2009·Current Microbiology·Hongxing ShenYang Zhou

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Citations

Nov 3, 2015·Journal of Molecular Biology·Thomas C Mettenleiter
Jun 25, 2016·Viruses·Inés Romero-Brey, Ralf Bartenschlager
Aug 3, 2016·Annual Review of Virology·Janna M Bigalke, Ekaterina E Heldwein
Dec 15, 2015·PloS One·Xiao-long DongMin-hui Pan
Nov 23, 2013·Biotechnology Progress·A Contreras-GómezY Chisti
Aug 14, 2020·MBio·Sailakshmi VelamoorMihnea Bostina

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