BMEC-1: a human bone marrow microvascular endothelial cell line with primary cell characteristics

Microvascular Research
F J CandalK L Kellar

Abstract

Bone marrow microvascular endothelial cells (BMEC) are a functional component of the bone marrow stroma and have been shown to release hematopoietic regulatory factors as well as to selectively adhere and support the proliferation and differentiation of CD34+ hematopoietic progenitors. An early passage of these cells was immortalized by transfection with a vector (pSVT) encoding the large T antigen of SV40. The transformed cell line (CDC/CU.BMEC-1) expresses the SV40 transcript, retains the primary cell expression of Ulex europeaus and vWF/ FVIII, and incorporates acetylated low-density lipoprotein. In addition, BMEC-1 mirrors the phenotype of the primary cells with only a few exceptions. Both cell populations express the cellular adhesion molecules ICAM-1 and PECAM and also VCAM-1 and ELAM-1 after upregulation by tumor necrosis factor-alpha. The fibronectin receptor, hyaluronate receptor, collagen receptor, integrins VLA-alpha 3, VLA-alpha 4, and beta 4, endoglin, collagen IV, CD58, and CD61 are also expressed. The only differences are that BMEC-1 expresses higher levels of ICAM-1, CD58, CD34, CD36, and c-kit than the primary cells. The supernatants of primary cell and BMEC-1 contain stem cell factor, interleukin-6 (IL-6), gra...Continue Reading

Citations

Jun 25, 2004·The American Journal of Pathology·Riccardo E NisatoMichael S Pepper
Jan 14, 1999·British Journal of Haematology·M H GauglerA Van der Meeren
Jul 25, 2000·European Journal of Clinical Investigation·P M RoodC E van der Schoot
Jun 18, 1999·Annals of the New York Academy of Sciences·R MöhleL Kanz
Sep 21, 2013·Disease Models & Mechanisms·Victoria W Wen, Karen L MacKenzie
Jan 24, 2013·PloS One·Mónica del ReyAlicia Rodríguez-Barbero
Feb 26, 1998·Leukemia & Lymphoma·S RafiiM A Moore
Jun 13, 2001·The Journal of Cell Biology·C J DimitroffR Sackstein
Oct 20, 2004·Journal of Cellular and Molecular Medicine·S W AdamsLaura F Gibson
Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maria Luisa Brandi, Patricia Collin-Osdoby
May 28, 2010·Journal of Cellular Biochemistry·Kang ChenZack Z Wang
Aug 11, 2000·In Vitro Cellular & Developmental Biology. Animal·U CavallaroR Montesano
Mar 7, 2001·Nature Biotechnology·J YangG S Herron
Dec 29, 2005·International Journal of Cancer. Journal International Du Cancer·R Daniel BonfilRafael Fridman
Jan 13, 2006·Journal of Cellular Biochemistry·Raphaële BuserMichael S Pepper
Sep 3, 1999·The Journal of Biological Chemistry·J YangG S Herron
Feb 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Niina VeitonmäkiYihai Cao
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andreas M BoehmlerRobert Möhle
Sep 21, 2017·Journal of Ovarian Research·Alon KedemZev Rosenwaks
Feb 19, 2004·Journal of Cell Science·Monia LazrakDanièle Mathieu
Jul 13, 2021·Frontiers in Cellular and Infection Microbiology·Luis Carlos Salazar AlvarezStefanie Costa Pinto Lopes

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