BMP-2 Enhances Lgr4 Gene Expression in Osteoblastic Cells

Journal of Cellular Physiology
Chantida Pawaputanon Na MahasarakhamMasaki Noda

Abstract

Osteoporosis is one of the most prevalent diseases and the number of patients suffering from this disease is soaring due to the increase in the aged population in the world. The severity of bone loss in osteoporosis is based on the levels of impairment in the balance between bone formation and bone resorption, two arms of the bone metabolism, and bone remodeling. However, determination of bone formation levels is under many layers of control that are as yet fully defined. Bone morphogenetic protein (BMP) plays a key role in regulation of bone formation while its downstream targets are still incompletely understood. Lgr4 gene encodes an orphan receptor and has been identified as a genetic determinant for bone mass in osteoporotic patients. Here, we examine the effects of BMP on the expression of Lgr4 in osteoblastic cells. Lgr4 gene is expressed in an osteoblastic cell line, MC3T3E1 in a time dependent manner during the culture. BMP treatment enhances Lgr4 mRNA expression at least in part via transcriptional event. When Lgr4 mRNA is knocked down, the levels of BMP-induced increase in alkaline phosphatase (Alp) activity and Alp mRNA are suppressed. BMP enhancement of Lgr4 gene expression is suppressed by FGF and reversed by dexam...Continue Reading

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Citations

Oct 18, 2016·Frontiers in Physiology·Bipradas RoyHugh M Fentress
Nov 20, 2016·Journal of Cellular Physiology·Chantida Pawaputanon Na MahasarakhamYoichi Ezura
Jan 11, 2018·Genes·Vitezslav Kriz, Vladimir Korinek
Mar 5, 2019·JBMR Plus·Vikram Khedgikar, Jessica A Lehoczky
Nov 9, 2019·International Journal of Molecular Sciences·Kazuhiro MaedaKeishi Marumo
May 14, 2020·Journal of Bone and Mineral Metabolism·Wei-Jia YuZhen-Lin Zhang
May 6, 2021·International Journal of Molecular Sciences·Alejandro Ordaz-RamosKarla Vazquez-Santillan

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