BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model

Materials Science & Engineering. C, Materials for Biological Applications
Ming-Kai HsiehPo-Liang Lai

Abstract

Gene therapy for bone tissue engineering has been widely developed. Recently, non-viral DNA-based gene therapy has been reported to be a safer and more efficient method of delivering DNA into target cells. We used a non-viral gene transfection reagent to delivery bone morphogenetic protein-2 (BMP-2) gene into bone marrow stromal cells (BMSCs). Primary BMSCs were isolated from rat femurs and transfected with BMP-2 plasmids. The transfection rate was analyzed using flow cytometry. The concentration of BMP-2 protein was quantified using an enzyme-linked immunosorbent assay. Levels of osteopontin and osteocalcin were measured to evaluate osteogenic differentiation. In vivo, we designed a critical-size calvarial defect rat model to study new bone regeneration, using Matrigel as a scaffold to carry BMP-2-transfected bone marrow stromal cells into the defect site. New bone formation was assessed by micro-computed tomography, X-ray, immunohistochemical staining and histomophometry. The transfection rate after 72 h was 31.5%. The BMP-2 protein level as well as osteopontin and osteocalcin expressions were higher in the experimental group (transfected with BMP-2) than the control group (transfected with green fluorescent protein, GFP). Th...Continue Reading

Citations

Nov 30, 2019·Nanomedicine·Michael J HillMorteza Mahmoudi
Jul 6, 2020·Journal of Tissue Engineering and Regenerative Medicine·Céline KleinRomuald Mentaverri
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Benjamin GantenbeinDevina Purmessur
Dec 9, 2019·ACS Biomaterials Science & Engineering·Lin ZhangShufang Wang

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