BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype

International Journal of Molecular Sciences
Xiaopeng ZhouQixin Chen

Abstract

Human mesenchymal stem cells (MSCs) have the potential to differentiate into nucleus pulposus (NP)-like cells under specific stimulatory conditions. Thus far, the effects of bone morphogenetic protein 3 (BMP3) and the cocktail effects of BMP3 and transforming growth factor (TGF)-β on MSC proliferation and differentiation remain obscure. Therefore, this study was designed to clarify these unknowns. MSCs were cultured with various gradients of BMP3 and BMP3/TGF-β, and compared with cultures in basal and TGF-β media. Cell proliferation, glycosaminoglycan (GAG) content, gene expression, and signaling proteins were measured to assess the effects of BMP3 and BMP3/TGF-β on MSCs. Cell number and GAG content increased upon the addition of BMP3 in a dose-dependent manner. The expression of COL2A1, ACAN, SOX9, and KRT19 increased following induction with BMP3 and TGF-β, in contrast to that of COL1A1, ALP, OPN, and COMP. Smad3 phosphorylation was upregulated by BMP3 and TGF-β, but BMP3 did not affect the phosphorylation of extracellular-signal regulated kinase (ERK) 1/2 or c-Jun N-terminal kinase (JNK). Our results reveal that BMP3 enhances MSC proliferation and differentiation into NP-like cells, as indicated by increased cell numbers and...Continue Reading

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Citations

Jun 28, 2016·International Journal of Molecular Sciences·Wilhelm Bloch
Nov 4, 2015·International Journal of Molecular Sciences·Zhe ZhangQiqing Zhang
Oct 4, 2019·Tissue Engineering. Part a·Daphne HingertHelena Brisby
Feb 21, 2019·Investigative Ophthalmology & Visual Science·Justin Ma, Peter Lwigale
Aug 18, 2018·Experimental and Therapeutic Medicine·Ying HeJinghong Chen
Aug 17, 2018·Experimental and Therapeutic Medicine·Numan KaraarslanOzkan Ates

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

QuantityOne
SPSS

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