BMP4-induced differentiation of a rat spermatogonial stem cell line causes changes in its cell adhesion properties

Biology of Reproduction
Gianfranco CarlomagnoAns M M van Pelt

Abstract

Spermatogonial stem cells (SSCs) are at the basis of the spermatogenic process and are essential for the continuous lifelong production of spermatozoa. Although several factors that govern SSC self-renewal and differentiation have been investigated, the direct effect of such factors on SSCs has not yet been studied, mainly because of the absence of markers to identify SSCs and the lack of effective methods to obtain and culture a pure population of SSCs. We now have used a previously established rat SSC cell line (GC-6spg) to elucidate the role of BMP4 in SSC differentiation. We found that GC-6spg cells cultured in the presence of BMP4 upregulate KIT expression, which is an early marker for differentiating spermatogonia. GC-6spg cells were found to express three BMP4 receptors and the downstream SMAD1/5/8 proteins were phosphorylated during BMP4-induced differentiation. A time-course DNA micro-array analysis revealed a total of 529 differentially regulated transcripts (≥2-fold), including several known downstream targets of BMP4 such as Id2 and Gata2. Pathway analysis revealed that the most affected pathways were those involved in adherens junctions, focal junctions, gap junctions, cell adhesion molecules, and regulation of act...Continue Reading

References

Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·L LarueR Kemler
Aug 1, 1996·Biology of Reproduction·A M van PeltM Stefanini
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
Feb 26, 2000·Science·F M Watt, B L Hogan
Dec 2, 2000·Differentiation; Research in Biological Diversity·A SomasiriC D Roskelley
Sep 22, 2001·Biology of Reproduction·H Chiarini-GarciaL D Russell
Feb 7, 2002·Genomics·Purvesh KhatriStephen A Krawetz
Apr 17, 2002·Endocrinology·Ans M M van PeltFederica M F van Dissel-Emiliani
Feb 28, 2003·Biology of Reproduction·Makoto NaganoRalph L Brinster
Mar 7, 2003·Genomics·Sorin DraghiciStephen A Krawetz
Mar 21, 2003·Developmental Biology·Duke DuguayMalcolm S Steinberg
Apr 18, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi KubotaRalph L Brinster
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Nov 13, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·H Chiarini-GarciaL D Russell
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christopher L TinkleElaine Fuchs
Apr 28, 2004·Developmental Biology·F Kent HamraDavid L Garbers
May 25, 2004·Nature Genetics·F William BuaasRobert E Braun
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi KubotaRalph L Brinster
Jan 27, 2005·Stem Cells·Marie-Claude HofmannMartyn Dym
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anne GorielyAndrew O M Wilkie
Jul 19, 2005·Nature Reviews. Molecular Cell Biology·Barry M Gumbiner
Aug 27, 2005·Developmental Biology·Rosa M BaleatoShaun D Roman
Aug 27, 2005·Oncogene·Jonas Larsson, Stefan Karlsson
Sep 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Buom-Yong RyuRalph L Brinster
Feb 17, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Pedro M AponteAns M M van Pelt
Oct 13, 2006·Biology of Reproduction·Masutaka TokudaTohru Marunouchi
Jun 8, 2007·Molecular Biology of the Cell·Robert W McLachlanAlpha S Yap
Mar 7, 2008·Journal of Cell Science·Margaret J WheelockKeith R Johnson
Jul 1, 2008·Annual Review of Cell and Developmental Biology·Jon M Oatley, Ralph L Brinster
Nov 6, 2008·Cell Stem Cell·Dirk G de RooijAns M M van Pelt
Nov 6, 2008·Cell Stem Cell·Mito Kanatsu-ShinoharaTakashi Shinohara

❮ Previous
Next ❯

Citations

Nov 1, 2011·Pharmacological Reviews·C Yan Cheng, Dolores D Mruk
Oct 9, 2013·Annual Review of Cell and Developmental Biology·Mito Kanatsu-Shinohara, Takashi Shinohara
Apr 5, 2012·Reproductive Biology·Agnieszka KolasaBarbara Wiszniewska
Nov 25, 2011·Reproduction in Domestic Animals = Zuchthygiene·T D LiuY J Wu
Mar 5, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yi LiJing Hao
Feb 10, 2012·Spermatogenesis·Lei ZhangYibing Han
Apr 11, 2014·Seminars in Cell & Developmental Biology·Sander van den DriescheRod T Mitchell
Mar 1, 2012·Biochimica Et Biophysica Acta·Sabrina Z JanTinke L Vormer
Dec 30, 2014·In Vitro Cellular & Developmental Biology. Animal·Lei ZhangYibing Han
Jan 20, 2018·Cytotechnology·Xiaoli ChenDong Wang
Feb 12, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Gurpreet Manku, Martine Culty
Dec 6, 2019·Journal of Assisted Reproduction and Genetics·Weiqiang ZhuRunsheng Li
Apr 17, 2018·Toxicology and Applied Pharmacology·Amélie R Tremblay, Geraldine Delbes
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Dai ZhouLiqing Fan

❮ Previous
Next ❯

Methods Mentioned

BETA
Infrared Imaging
in vitro transcription

Software Mentioned

Pathway - Express from
Tools
Onto
Genespring GX
- Express
Tools Pathway - Express
Graphpad
Pathway

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.