Abstract
Bone morphogenetic protein (BMP)9 has been reported to be the most potent BMP to induce bone formation. However, the details of BMP9-transduced intracellular signaling remain ambiguous. Here, we have investigated signal transduction mechanisms of BMP9 in comparison to BMP2, another potent inducer of bone formation, in osteoblasts. In a mouse osteoblast cell line, BMP9 induced higher mRNA levels of alkaline phosphatase (ALP) and runt-related transcription factor 2 (Runx2) than BMP2 within 2 h. Unlike BMP2, BMP9 induced rapid phosphorylation of glycogen synthase kinase 3-β (GSK3-β) and protein kinase B (Akt) and increased the cellular protein content of β-catenin. BMP9 moderately increased mRNA levels of several canonical Wingless-related integration site to lower degrees than BMP2. Furthermore, BMP9-induced GSK3-β phosphorylation was not inhibited by pretreatment with actinomycin D, cycloheximide, or Brefeldin A, indicating it is independent of Wnt protein secretion. BMP9-induced GSK3-β phosphorylation was abrogated by Akt or class I PI3K-specific inhibitors. Moreover, inactivation of GSK3-β by LiCl did not further promote ALP and Runx2 mRNA induction by BMP9 as significantly as that by BMP2. Notably, BMP9-induced GSK3-β phospho...Continue Reading
References
Aug 14, 1992·Biochemical and Biophysical Research Communications·K OkamuraM Kuwano
Aug 1, 1994·Endocrine Reviews·M P Whyte
Dec 21, 1995·Nature·D A CrossB A Hemmings
Nov 24, 2001·Cell·Y GongUNKNOWN Osteoporosis-Pseudoglioma Syndrome Collaborative Group
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Hongwei ChengTong-Chuan He
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Georges RawadiSergio Roman-Roman
Jul 23, 2004·Gene Therapy·Q KangT-C He
Sep 24, 2004·The Journal of Biological Chemistry·Youn-Jeong KimHyun-Mo Ryoo
Apr 27, 2005·The Journal of Biological Chemistry·Monica A BrownSenyon Choe
Feb 16, 2006·Journal of Orthopaedic Trauma·Joseph M Lane
Jul 28, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Etsuko MatsuzakiToshiyuki Sasaguri
Feb 22, 2007·Journal of Cell Science·Marion ScharpfeneckerP ten Dijke
Apr 3, 2008·Journal of Tissue Engineering and Regenerative Medicine·P C BessaR L Reis
Jul 12, 2008·Stem Cells and Development·Quan KangTong-Chuan He
Jul 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zahia HamidoucheOlivia Fromigué
Nov 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tetsuya MatsuguchiTomokazu Ohnishi
Apr 25, 2009·Tissue Engineering. Part a·Eric BergeronNathalie Faucheux
Oct 28, 2009·Bone·Rishard SalieMira Susa
Jul 16, 2010·The Journal of Biological Chemistry·Jinyong LuoTong-Chuan He
Oct 21, 2010·Cellular Signalling·Beth BragdonAnja Nohe
May 26, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Elisabeth LeblancGuillaume Grenier
Jun 29, 2011·Cellular and Molecular Life Sciences : CMLS·Marie BidartSabine Bailly
Jul 9, 2011·The Journal of Biological Chemistry·Roselyne CastonguayAsya V Grinberg
May 18, 2012·Molecular Biology of the Cell·Nam Y LeeGerard C Blobe
Jun 8, 2012·Endocrine Journal·Masato YanoHiroshi Kaji
Jun 22, 2012·The Journal of Biological Chemistry·Sharon A TownsonAsya V Grinberg
Mar 26, 2013·The Journal of Clinical Investigation·Hong OuyangKang Zhang
Sep 25, 2014·Current Topics in Developmental Biology·David WeberManfred Gessler
Jan 17, 2015·Trends in Cell Biology·Derek P BrazilFinian Martin
Feb 25, 2015·Mediators of Inflammation·Łukasz A PoniatowskiDariusz Szukiewicz
Mar 19, 2016·Clinical Oral Implants Research·Masako Fujioka-KobayashiRichard J Miron
Jun 1, 2017·Cell Reports·Takako SaitoLuca Jovine
Citations
Dec 10, 2019·American Journal of Reproductive Immunology : AJRI·Narmada LavuRamkumar Menon
Jun 9, 2020·Biochemical Society Transactions·Liu LiuDingming Huang
Jan 31, 2020·Cell Death & Disease·Yuwan LiWei Huang
Dec 3, 2020·FEBS Letters·Chang-Hwan SeongTetsuya Matsuguchi
Dec 15, 2020·Stem Cells and Development·Charles Owen Smith, Roman A Eliseev
Feb 10, 2021·Cancer Cell International·Qiuqiu ZhangHan Zhang
Mar 9, 2021·Oral Diseases·Na Young ShimJung Sun Heo
Mar 24, 2021·Clinical Oral Investigations·Takatomo ImafujiKazuyuki Noguchi
Dec 24, 2020·Human Molecular Genetics·Wenhui YuHuiyong Shen
Nov 28, 2021·Aging·Ziming LiuYingfang Ao