BMSCs and miR-124a ameliorated diabetic nephropathy via inhibiting notch signalling pathway

Journal of Cellular and Molecular Medicine
Jiping SunAiping Yin

Abstract

BMSCs are important in replacement therapy of diabetic nephropathy (DN). MiR-124a exerts effect on the differentiation capability of pancreatic progenitor cells. The objective of this study was to explore the molecular mechanisms, the functions of miR-124a and bone marrow mesenchymal stem cells (BMSCs) in the treatment of DN. Characterizations of BMSCs were identified using the inverted microscope and flow cytometer. The differentiations of BMSCs were analysed by immunofluorescence assay and DTZ staining. The expression levels of islet cell-specific transcription factors, apoptosis-related genes, podocytes-related genes and Notch signalling components were detected using quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot assays. The production of insulin secretion was detected by adopting radioimmunoassay. Cell proliferation and apoptosis abilities were detected by CCK-8, flow cytometry and TUNEL assays. We found that BMSCs was induced into islet-like cells and that miR-124a could promote the BMSCs to differentiate into islet-like cells. BMSCs in combination with miR-124a regulated islet cell-specific transcription factors, apoptosis-related genes, podocytes-related genes as well as the activity of Notc...Continue Reading

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Citations

Mar 2, 2019·Diabetology & Metabolic Syndrome·Mairim Alexandra SolisLynn L H Huang
Jan 7, 2020·Journal of the Chinese Medical Association : JCMA·Xia NiuJiulai Tang
Nov 23, 2020·Cell Death & Disease·Dongwei LiuZhangsuo Liu
Feb 2, 2021·Frontiers in Pharmacology·Carlamaria ZojaDaniela Macconi
Jun 10, 2021·Stem Cells Translational Medicine·LaTonya J HicksonM Hassan Murad
Jun 20, 2021·Clinical and Experimental Nephrology·Ningmin ZhaoPeizhi Ma
Jun 25, 2021·Frontiers in Endocrinology·Yi WangLing-Qing Yuan
Jan 8, 2022·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ling JiangYonggui Wu

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Methods Mentioned

BETA
FACS
transfection
transfecting
flow cytometry
reverse transcription PCR
PCR
fluorescence microscopy

Software Mentioned

CellQuest
Lab Works Image Acquisition and Analysis

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