BNC1 Promotes Spermatogenesis by Regulating Transcription of Ybx2 and Papolb via Direct Binding to Their Promotor Elements.

Reproductive Sciences
Jing-Yi LiDan Zhang

Abstract

BNC1 is a transcription factor that is crucial for spermatogenesis and male fertility, although the underlying mechanism remains unclear. To study BNC1's specific role in spermatogenesis, we characterized a previously developed mouse model carrying a truncating mutation in Bnc1 (termed Bnc1+/tr for heterozygotes and Bnc1tr/tr for homozygotes) and found that the mutation decreased BNC1 protein levels and resulted in germ cell loss by apoptosis. Given that loss of functional Bnc1 is known to result in decreased expression of the spermatogenesis genes Ybx2 and Papolb, we aimed to explore whether and how BNC1 promotes transcription of Ybx2 and Papolb to mediate its role in spermatogenesis. We confirmed significant reduction in YBX2 and PAPOLB protein levels in testis tissue from Bnc1+/tr and Bnc1tr/tr males compared with wild-type mice (Bnc1+/+). Consistently, knockdown of Bnc1 led to downregulation of Ybx2 and Papolb in CRL-2196 cells in vitro. To investigate if BNC1 directly induces Ybx2 and Papolb gene expression, chromatin immunoprecipitation using mouse testicular tissue and luciferase reporter assays in HEK293 cells were used to identify functional binding of BNC1 to the Ybx2 and Papolb promoters at defined BNC1 binding sites...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H Tseng, H Green
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·M C HofmannJ L Millán
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·S Iuchi, H Green
May 1, 2004·The Journal of Reproduction and Development·Tiangang ZhuangTadashi Baba
Jan 27, 2005·Stem Cells·Marie-Claude HofmannMartyn Dym
Jan 18, 2006·Molecular and Cellular Endocrinology·Juxiang YangNorman B Hecht
Aug 22, 2006·Biochemical and Biophysical Research Communications·Junwen WangHung Tseng
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anilkumar Bettegowda, Miles F Wilkinson
Jan 24, 2012·Genesis : the Journal of Genetics and Development·Xiaohong ZhangHung Tseng
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yueh-Chiang HuDavid C Page
Oct 23, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Danielle L CullinaneKenneth C Kleene
Sep 21, 2016·The Journal of Reproduction and Development·Shin-Ichi KashiwabaraTadashi Baba
Nov 8, 2017·The Journal of Reproduction and Development·Shin-Ichi KashiwabaraTadashi Baba
Jul 17, 2018·Human Molecular Genetics·Dan ZhangHefeng Huang
Aug 11, 2018·The Journal of Cell Biology·Marina VolinHila Toledano

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