Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae

The EMBO Journal
H ImamuraY Takai

Abstract

The RHO1 gene encodes a homologue of mammalian RhoA small G-protein in the yeast Saccharomyces cerevisiae. Rho1p is required for bud formation and is localized at a bud tip or a cytokinesis site. We have recently shown that Bni1p is a potential target of Rho1p. Bni1p shares the FH1 and FH2 domains with proteins involved in cytokinesis or establishment of cell polarity. In S. cerevisiae, there is an open reading frame (YIL159W) which encodes another protein having the FH1 and FH2 domains and we have named this gene BNR1 (BNI1 Related). Bnr1p interacts with another Rho family member, Rho4p, but not with Rho1p. Disruption of BNI1 or BNR1 does not show any deleterious effect on cell growth, but the bni1 bnr1 mutant shows a severe temperature-sensitive growth phenotype. Cells of the bni1 bnr1 mutant arrested at the restrictive temperature are deficient in bud emergence, exhibit a random distribution of cortical actin patches and often become multinucleate. These phenotypes are similar to those of the mutant of PFY1, which encodes profilin, an actin-binding protein. Moreover, yeast two-hybrid and biochemical studies demonstrate that Bni1p and Bnr1p interact directly with profilin at the FH1 domains. These results indicate that Bni1p ...Continue Reading

References

Aug 15, 1976·Journal of Molecular Biology·L CarlssonS Lövgren
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Sep 2, 1985·European Journal of Biochemistry·M Tanaka, H Shibata
Jun 1, 1995·Trends in Biochemical Sciences·Y TakaiH Nakanishi
Apr 1, 1995·Current Opinion in Cell Biology·D E Levin, B Errede
Jul 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R H Sohn, P J Goldschmidt-Clermont
Apr 19, 1996·The Journal of Biological Chemistry·Y KamadaD E Levin
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·R KolluriT Kirchhausen

❮ Previous
Next ❯

Citations

May 28, 2013·Molecular Genetics and Genomics : MGG·Mitsunori YoshidaYoshikazu Ohya
Nov 1, 2003·Biochemical and Biophysical Research Communications·Patricia M Bergamo FavaroSara Teresinha Olalla Saad
Dec 4, 2003·Fungal Genetics and Biology : FG & B·Gretel M GuestMichelle Momany
Jun 14, 2005·Trends in Biochemical Sciences·Henry N Higgs
May 10, 2005·Experimental Cell Research·Judith E GasteierOliver T Fackler
Jan 13, 1998·Current Opinion in Cell Biology·M Glotzer
Mar 4, 1998·Current Opinion in Cell Biology·K R Ayscough
Feb 27, 1999·Current Opinion in Cell Biology·C FieldK Oegema
Feb 27, 1999·Current Opinion in Cell Biology·J D Sutherland, W Witke
May 10, 2000·Trends in Cell Biology·D N Robinson, J A Spudich
Jul 31, 2003·Trends in Cell Biology·Bradley J Wallar, Arthur S Alberts
May 1, 1998·Trends in Cardiovascular Medicine·M AmanoK Kaibuchi
Apr 6, 2002·Current Opinion in Microbiology·Antonio Casamayor, Michael Snyder
Nov 30, 2002·Current Opinion in Microbiology·Daniel SchottAnthony Bretscher
Mar 6, 1999·Current Opinion in Microbiology·S J Palmieri, B K Haarer
Dec 3, 2003·Nature Cell Biology·Agnieszka KobielakElaine Fuchs
Oct 24, 2008·Nature Reviews. Molecular Cell Biology·Rong Li, Gregg G Gundersen
Dec 10, 2009·Nature Reviews. Molecular Cell Biology·Melissa A ChesaroneBruce L Goode
May 19, 2000·The Biochemical Journal·A L Bishop, A Hall
Dec 17, 2004·Biology of the Cell·John Condeelis, Robert H Singer
Aug 13, 2011·The Journal of Biological Chemistry·Sapan BorahRupinder Kaur
Feb 21, 2002·Molecular Biology of the Cell·Kathryn E Sharpless, Steven D Harris
Feb 26, 2000·Molecular Biology of the Cell·E A VallenE Bi
Feb 18, 2003·Molecular Biology of the Cell·Kenjiro MisuKazuma Tanaka
Sep 17, 2004·Molecular Biology of the Cell·David PruyneAnthony Bretscher
Oct 21, 2005·Molecular Biology of the Cell·Hans-Peter SchmitzPeter Philippsen
Mar 9, 2007·Molecular Biology of the Cell·Shawnna M ButteryDavid Pellman
Feb 27, 2009·Molecular Biology of the Cell·Colleen T SkauDavid R Kovar
Dec 15, 2010·Molecular Biology of the Cell·Felipe O Bendezú, Sophie G Martin
Aug 25, 2012·Molecular Biology of the Cell·Shawnna M ButteryDavid Pellman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.