BNIP3 as a Regulator of Cisplatin-Induced Apoptosis.

Biochemistry. Biokhimii︠a︡
Anna S GorbunovaBoris Zhivotovsky

Abstract

BNIP3 is a member of Bcl-2 protein family involved in regulation of various forms of cell death. However, its role in these processes remains unclear and varies depending on the type of cancer cells and environmental factors (pH, O2 level, etc.). Here, the role of BNIP3 in apoptosis regulation in lung adenocarcinoma cells was investigated. The suppressed expression of BNIP3 caused inhibition of oxygen consumption and stimulated production of the mitochondrial reactive oxygen species, suggesting the role of BNIP3 in induction of mitochondrial dysfunction and its potential involvement in regulation of cell death. Indeed, cytochrome c release in the cells with BNIP3 knockout and knockdown was higher than in the wild-type (WT) upon apoptosis stimulation by cisplatin. Moreover, suppression of BNIP3 expression led to the increase in the caspase-3 activity and, as a consequence, accumulation of the apoptotic marker - p89 fragment of poly(ADP-ribose)-polymerase (PARP) - as compared to WT cells. Analysis of the SubG1 population by flow cytometry confirmed the elevated level of apoptosis in the BNIP3 knockout cells. Pretreatment with the antioxidant Trolox did not affect cell death, indicating that it was independent on reactive oxygen s...Continue Reading

References

Jun 1, 1994·Free Radical Biology & Medicine·V J ForrestN Ramakrishnan
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Feb 9, 2000·Journal of Bioenergetics and Biomembranes·J Cai, D P Jones
May 23, 2002·Biochimie·Christophe FleuryJean-Luc Vayssière
Jun 11, 2004·Annual Review of Biochemistry·Xuejun Jiang, Xiaodong Wang
Feb 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gerald SchwerdtMichael Gekle
Feb 21, 2009·Cell Death and Differentiation·J Zhang, P A Ney
Jul 10, 2009·Autophagy·Nathalie M Mazure, Jacques Pouysségur
Sep 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Youngil LeeÅsa B Gustafsson
Apr 18, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jie ZhengWei-Qun Ding
Jul 25, 2014·European Journal of Pharmacology·Shaloam Dasari, Paul Bernard Tchounwou
Aug 2, 2015·EMBO Reports·Aparajita H ChourasiaKay F Macleod
Sep 1, 2015·Autophagy·Aparajita H Chourasia, Kay F Macleod
Jun 21, 2018·Biological Chemistry·Alibek AbdrakhmanovVladimir Gogvadze
Jun 19, 2019·Oxidative Medicine and Cellular Longevity·Enza VernucciMatteo A Russo
Jul 13, 2019·International Journal of Molecular Sciences·Veronica CocettaMonica Montopoli

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
Protein Assay
FACS

Software Mentioned

Seahorse Analyzer

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis