Dec 1, 1975

Boar acrosin, II: Amino acid composition, amino terminal residue and molecular weight estimations by ultracentrifugation

Hoppe-Seyler's Zeitschrift für physiologische Chemie
W D SchleuningH Fritz

Abstract

The molecular weight of boar acrosin in neutral solution was estimated to be 41000 +/- 1000 by high-speed sedimentation equilibrium analysis. This result is in good agreement with the value found earlier[1] by sodium dodecylsulfate polyacrylamide gel electrophoresis. The sedimentation coefficeint of acrosin obtained by active enzyme centrifugation of partly purified preparations is in accordance with the sedimentation coefficient of the pure preparation estimated by conventional sedimentation velocity analysis. The sedimentation coefficient of acrosin is considerably decreased in slightly acidic solution (pH 4), indicating that changes in the tertiary structure occur upon acidification. The amino acid composition of the acrosin preparation homogeneous by electrophoretic and chromatographic criteria and in sedimentation studies was determined. Valine was found as the unique N-terminal amino acid. However, in microheterogeneous forms of acrosin, alanine and methionine were also detected in end group analysis.

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Mentioned in this Paper

Benzoylarginine Nitroanilide, Monosodium Salt, Monohydrochloride
Endopeptidases
M beta-Acrosin
ACR
Gamma-Thrombin
Ultracentrifugation
Hydrogen-Ion Concentration
Trypure

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