PMID: 1526Dec 1, 1975

Boar seminal zinc-precipitable protein and the haemagglutinin

Journal of Reproduction and Fertility
J C BoursnellM G Andrews

Abstract

About 30% of boar seminal plasma nitrogen is maximally precipitated at room temperature by 6 to 10 mM zinc in citrate solution at pH 8. A rise in the total nitrogen precipitated by 1 to 6 mM zinc is accompanied by a fall in the haemagglutinin titre of the supernatant fluid. At 6 mM zinc addition, 95% of the haemagglutinin is precipitated, but much of this is recoverable by re-solution of the zinc precipitate. Protein profile studies by gel-filtration chromatography of the zinc precipitate solution reveals a mixture of proteins, some of which are not by themselves zinc-precipitable.

Citations

Dec 12, 2012·Polish Journal of Veterinary Sciences·M Mogielnicka-BrzozowskaW Kordan

Related Concepts

Agglutinins
Molecular Sieve Chromatography
Isohemagglutinins
Hydrogen-Ion Concentration
Nitrogen
Seminal Plasma
Zinc

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