PMID: 7539216May 1, 1995Paper

Boar sperm plasma membrane Ca(2+)-selective channels in planar lipid bilayers

The American Journal of Physiology
S K Tiwari-Woodruff, T C Cox

Abstract

Entry of Ca2+ through Ca2+ channels is thought to trigger the acrosome reaction of spermatozoa during fertilization. Antagonists of the L-type Ca2+ channel are known to prevent the intracellular Ca2+ (Ca2+) increase and inhibit acrosomal exocytosis in mammalian sperm. Planar bilayer recordings were used to study Ca2- channels incorporated from partially purified boar sperm plasma membranes. With symmetrical 50 mM NaCl and 100 mM BaCl2 on the cis side, single-channel events consistent with Ba2+ flux from cis to trans were observed. These channels were activated by the dihydropyridine agonist (+/-)BAY K 8644 and blocked by the antagonist nitrendipine. Sperm Ca2- channels did not require depolarization for activation and did not inactivate. The (+/-)BAY K 8644 and (S-)BAY K 8644 enantiomers increased apparent open time in a dose-dependent [half-maximal activity constant (K0.5) = 0.9 and 0.3 microM, respectively] manner. Dihydropyridine antagonists nitrendipine (K0.5 = 0.5 microM) and (R+)BAY K 8644 (K0.5 = 2.8 microM) decreased apparent open times. The channels described in this report share some properties with brain, cardiac, and skeletal muscle t tubule Ca2+ channels and may be involved in increasing Cai2+ before the acrosome r...Continue Reading

References

Oct 1, 1991·Molecular Reproduction and Development·T CoxR N Peterson
Feb 1, 1991·The Journal of General Physiology·R Mejía-AlvarezE Stefani
Feb 1, 1990·The Journal of General Physiology·A LiévanoA Darszon
Aug 1, 1986·Journal of Muscle Research and Cell Motility·A J Avila-SakarE Stefani
May 30, 1989·Biochemical and Biophysical Research Communications·T Cox, R N Peterson
Sep 1, 1984·The Journal of Physiology·K S Lee, R W Tsien
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B P Bean
Oct 11, 1984·Nature·A M BrownA Yatani
Jan 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C D Berdanier

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Citations

Feb 18, 2004·The Journal of Reproduction and Development·Qing-Yuan Sun, Takashi Nagai
Oct 31, 2019·Human Reproduction Update·Sean G BrownSarah J Martins da Silva
Jun 11, 1998·Molecular Reproduction and Development·Y L Shi, X H Ma
Apr 30, 1999·Physiological Reviews·A DarszonF Espinosa

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