Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation

Theriogenology
C DubéJ L Bailey

Abstract

Androhep Plus, a long-term extender (up to 7 days) and Beltsville Thawing Solution (BTS), a short-term extender (up to 3 days), are commonly used for liquid storage of porcine semen. To test the hypothesis that modifications in sperm viability, motility, chlortetracycline (CTC) fluorescence patterns, and protein tyrosine phosphorylation occur during semen storage in extenders, we compared these end points at different periods of storage in either Androhep Plus or BTS. Sperm from five boars were assessed daily over 12 days of storage (n = 5 ejaculates from different boars). Viability was not different (P < 0.05 between extenders, except on Day 2, when Androhep Plus maintained better viability. Differences in the percentage of motile (total) sperm due to extender were evident on Days 2, 4, 5, and 6, when Androhep Plus was superior to BTS (P < 0.05). The percentages of progressively motile sperm also differed, with Androhep Plus supporting higher rates on Days 2, 4, 5, 7, 8, 9, 10, and 11 (P < 0.05). The CTC fluorescence pattern distribution differed due to extender as early as Day 2; storage in Androhep Plus induced higher levels of pattern B sperm (P < 0.05) than storage in BTS. A tyrosine-phosphorylated protein of Mr 21,000 app...Continue Reading

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Citations

Jan 14, 2005·The Journal of Reproduction and Development·Tetsuma MuraseToshio Tsubota
Nov 21, 2008·Reproduction in Domestic Animals = Zuchthygiene·C-L XuJ-H Tan
Sep 9, 2011·Reproduction in Domestic Animals = Zuchthygiene·D WaberskiA M Petrunkina
Mar 9, 2018·Reproduction, Fertility, and Development·William V Holt, Nana Satake
Sep 26, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Erik PiehlerEdda Töpfer-Petersen
Nov 12, 2018·Reproduction, Fertility, and Development·Santiago T PeñaDamien B B P Paris
May 18, 2019·Reproduction in Domestic Animals = Zuchthygiene·Na WangJia-Bo Zhou
Sep 9, 2011·Reproduction in Domestic Animals = Zuchthygiene·L RadomilM M Buhr
Aug 31, 2019·Scientific Reports·Michal ZigoPeter Sutovsky
Jul 16, 2020·Scientific Reports·Won-Ki PangMyung-Geol Pang
Sep 13, 2017·Acta Veterinaria Scandinavica·Wiesław BielasRyszard Mordak
Dec 24, 2019·Frontiers in Veterinary Science·Isabel BarrancoInmaculada Parrilla

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