Bob1 (OCA-B/OBF-1) differential transactivation of the B cell-specific B29 (Ig beta) and mb-1 (Ig alpha) promoters

The Journal of Immunology : Official Journal of the American Association of Immunologists
C S Malone, R Wall

Abstract

The B29 (Igbeta) and mb-1 (Igalpha) gene products are B cell-specific essential components of the B cell receptor that are coexpressed at all stages of B cell differentiation, with the exception of plasma cells, which lack mb-1 expression. Transcription of both genes is governed by a similar cassette of interactive transcription factor-binding elements, including octamer motifs, in TATA-less promoters. In this study, we show the B cell-specific B29 gene promoter is transactivated in B and non-B cells by cotransfection with the B cell-specific octamer cofactor gene, Bob1 (OCA-B/OBF-1). The expression of Bob1 is also sufficient to override the silencing effects of the B29 silencer. This indicates that Bob1 plays a critical role in B cell-specific B29 promoter expression. In contrast, coexpression of Bob1 had no effect on mb-1 promoter activity. Bob1 transactivation only occurs with select octamer sequences that have an adenosine at position 5 (ATGCAAAT). The B29 promoter conforms to this consensus octamer motif, while the mb-1 promoter octamer motif does not. Octamer motif swapping between B29 and mb-1 promoters renders B29 unresponsive to Bob1 transactivation and makes mb-1 competent for Bob1 transactivation, thereby indicating ...Continue Reading

References

Mar 1, 1992·Molecular and Cellular Biology·A L FeldhausH Singh
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G G HermansonR Wall
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G G HermansonR Wall
Jan 26, 1995·Nature·M GstaigerC M Hovens
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S A Omori, R Wall
Jun 1, 1993·Molecular and Cellular Biology·A TravisR Grosschedl
Dec 15, 1995·The Journal of Biological Chemistry·P PfistererT Wirth
Jun 21, 1996·Science·P E Roth, A L DeFranco
Aug 15, 1996·Genes & Development·K L CepekP A Sharp
Dec 1, 1996·European Journal of Immunology·P J NielsenW Schaffner
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·C S MaloneR Wall
Dec 22, 1998·Molecular and Cellular Biology·P AkerbladM Sigvardsson
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C S MaloneR Wall
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S StevensR G Roeder
Sep 23, 2000·Molecular Immunology·C S MaloneR Wall

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Citations

Jan 14, 2003·Annual Review of Immunology·Kathryn L CalameChainarong Tunyaplin
Nov 26, 2002·Molecular and Cellular Biology·Mikael SigvardssonJames Hagman
Aug 30, 2003·The Journal of Biological Chemistry·Cornelia BrunnerThomas Wirth
Nov 18, 2006·European Journal of Immunology·Christian VettermannHans-Martin Jäck
May 8, 2013·The Journal of Biological Chemistry·Eung Jae YooStephen A Liebhaber
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melinda S GordonRandolph Wall
Jul 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Boris BartholdyPatrick Matthias
Feb 12, 2021·The Journal of Allergy and Clinical Immunology·Patrick KuryMarta Rizzi
Aug 22, 2006·Journal of Molecular Biology·Cindy S MaloneRandolph Wall
Nov 25, 2020·Molecular Cell·Chi-Shuen ChuRobert G Roeder

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Proceedings of the National Academy of Sciences of the United States of America
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