BODIPY-loaded polymer nanoparticles: chemical structure of cargo defines leakage from nanocarrier in living cells

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Kateryna TrofymchukAndrey S Klymchenko

Abstract

Uncontrolled release of encapsulated drugs and contrast agents from biodegradable polymer nanoparticles (NPs) is a central problem in drug delivery and bioimaging. In particular, it concerns polymeric NPs prepared by nanoprecipitation, where this release (so-called burst release) can be very significant, leading to side effects and/or bioimaging artifacts. Here, we systematically studied the effect of the chemical structure of cargo molecules, BODIPY dye derivatives, on their capacity to be loaded into ∼50 nm PLGA NPs without leakage in biological media. Absorption and fluorescence spectroscopy suggested that all the dyes, except the most polar BODIPY derivative, formed blended structures with polymer NPs. Fluorescence correlation spectroscopy of dye-loaded NPs in the presence of serum proteins revealed that only the most hydrophobic BODIPY dyes, bearing one octadecyl chain or two octyl chains, remain inside the NPs, while all other derivatives are released into the serum medium. The time-lapse absorption and fluorescence studies confirmed this result, suggesting the release kinetics for the leaky NPs on the time scale of hours. Fluorescence microscopy of living cells incubated with BODIPY-loaded NPs showed that most of them ex...Continue Reading

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Citations

Oct 2, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wenkun HanWenjing Tian
Nov 14, 2019·Pharmaceutics·Maria Camilla OpertiOya Tagit
Apr 6, 2021·Nano Letters·Man Theerasilp, Daniel Crespy
Jun 10, 2021·ACS Applied Materials & Interfaces·Anila Hoskere Ashoka, Andrey S Klymchenko
Jun 25, 2021·Chemistry : a European Journal·Bohdan AndreiukAndrey S Klymchenko
Aug 13, 2021·Journal of the American Chemical Society·Cheyenne J ChristophersonZachary M Hudson
Dec 25, 2019·ACS Applied Materials & Interfaces·Anne RunserAndreas Reisch
Sep 25, 2021·International Journal of Pharmaceutics·Yanbing WangFeng Gao
Nov 3, 2021·The Journal of Organic Chemistry·William H García-SantosAlejandro Cordero-Vargas

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Methods Mentioned

BETA
fluorescence imaging
nanoprecipitation
florescence correlation spectroscopy
fluorescence microscopy
dynamic light scattering
fluorescence correlation spectroscopy
FCS
confocal microscopy
optical spectroscopy
fluorescence studies

Software Mentioned

Zetasizer
PyCorrFit

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