Body-barrier surveillance by epidermal γδ TCRs.

Nature Immunology
Grzegorz ChodaczekTomasz Zal

Abstract

The surveillance of body barriers relies on resident T cells whose repertoires are biased toward particular γδ T cell antigen receptors (TCRs) according to location. These γδ TCRs can recognize ligands that emerge after stress. Through the use of intravital dynamics-immunosignal correlative microscopy, we found that γ-chain variable region 5 (V(γ)5) TCRs expressed by epidermal T cells were constitutively clustered and functionally activated in vivo at steady state, forming true immunological synapses that polarized and anchored T cell projections at squamous keratinocyte tight junctions. This synaptogenesis depended on TCR variable domains, the kinase Lck and the integrin α(E)β(7) but not the γδ lineage or the receptor NKG2D. In response to tissue stress, TCR-proximal signals did not increase substantially but underwent stress mode-dependent relocalization toward the basal epidermis and Langerhans cells. Thus, the γδ TCR orchestrates barrier surveillance proactively, presumably by recognizing tissue ligands expressed in the steady state.

References

Mar 6, 1992·Cell·P MombaertsV E Papaioannou
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·H KomanoS Itohara
Jan 14, 1994·Cell·K A HogquistF R Carbone
Feb 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R G Parton
Nov 1, 1996·The Journal of Investigative Dermatology·M MohamadzadehA Takashima
Jul 29, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W MöbiusG Schwarzmann
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H HaraK Nomoto
Sep 22, 2001·Science·M GirardiA C Hayday
Apr 27, 2002·Science·Julie JamesonWendy L Havran
May 30, 2002·Nature Reviews. Immunology·Simon R Carding, Paul J Egan
Sep 24, 2002·Nature Immunology·Benjamin A FreibergAbraham Kupfer
Oct 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yaping ChenRichard Boismenu
Mar 21, 2003·European Journal of Immunology·Andreas DiefenbachDavid H Raulet
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie M JamesonWendy L Havran
Apr 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mary A YuiEllen V Rothenberg
Oct 12, 2005·The Journal of Experimental Medicine·Gabriele CampiMichael L Dustin
Jan 28, 2006·The Journal of Investigative Dermatology·Michael GirardiRobert E Tigelaar
Feb 13, 2007·Immunological Reviews·Na Xiong, David H Raulet
Oct 6, 2007·Biochimica Et Biophysica Acta·Hideki ChibaNorimasa Sawada
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Jan 6, 2009·Immunological Reviews·Lewis L Lanier
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael I WhangDavid H Raulet
Dec 10, 2009·The Journal of Experimental Medicine·Akiharu KuboMasayuki Amagai
Sep 17, 2010·Cold Spring Harbor Perspectives in Biology·Michael L DustinAndrey S Shaw
Nov 30, 2010·FEBS Letters·Takashi SaitoAkiko Hashimoto-Tane
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Susannah D BarbeeAdrian C Hayday

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Citations

Sep 14, 2013·Seminars in Immunopathology·Jörg Stange, Marc Veldhoen
Jan 9, 2013·Immunology and Cell Biology·Scott N Mueller
Feb 22, 2012·Nature Immunology·Adrian Hayday, Robert Tigelaar
Aug 23, 2012·Nature Immunology·Deborah A Witherden, Wendy L Havran
Apr 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen L EdelblumJerrold R Turner
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Silvia AriottiTon N Schumacher
Sep 26, 2012·The Cancer Journal·Michael Youngjun Choi, Mary Jo Lechowicz
Sep 21, 2013·The Journal of Clinical Investigation·Amanda S MacLeodWendy L Havran
Mar 4, 2014·Current Dermatology Reports·Szun S TayWolfgang Weninger
Feb 28, 2014·Seminars in Immunopathology·Tim Lämmermann, Ronald N Germain
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ali ZaidScott N Mueller
Jul 18, 2014·Advances in Wound Care·Natasa StrboOlivera Stojadinovic
Oct 7, 2014·Trends in Immunology·Adrienne M LuomaErin J Adams
Jan 26, 2013·Nature Reviews. Immunology·Pierre Vantourout, Adrian Hayday
Apr 27, 2013·Journal of Leukocyte Biology·Deborah A Witherden, Wendy L Havran
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Dibyendu Kumar DasMatthew J Lang
Jul 19, 2012·European Journal of Immunology·Chiara RigantiMatthias Eberl
Apr 3, 2016·Cellular and Molecular Life Sciences : CMLS·Hongmei FuFederica M Marelli-Berg
Feb 5, 2015·Cellular Immunology·Rebecca L O'Brien, Willi K Born
May 11, 2015·Cellular Immunology·Kevin RamirezWendy L Havran
Mar 18, 2015·Immunology and Cell Biology·Sioh-Yang TanWolfgang Weninger
Aug 1, 2014·Frontiers in Immunology·Scott N MuellerFrancis R Carbone
Dec 17, 2014·Frontiers in Immunology·Deborah A WitherdenWendy L Havran
Jan 8, 2015·Frontiers in Immunology·Drew C DenigerLaurence J N Cooper
Aug 15, 2014·Frontiers in Immunology·Alexandre P BenechetKamal M Khanna
Feb 6, 2015·Frontiers in Immunology·Lionel CouziJulie Déchanet-Merville
Jun 9, 2016·The Journal of Experimental Medicine·Sara McArdleKlaus Ley
Oct 28, 2016·The Journal of Investigative Dermatology·Jutamas SuwanpradidAmanda S MacLeod
Nov 8, 2016·The Journal of Clinical Investigation·Kiwon ParkCatherine C Hedrick
Nov 18, 2016·Pflügers Archiv : European journal of physiology·Katja Bäsler, Johanna M Brandner
Aug 26, 2014·Immunity·Nadia Kurd, Ellen A Robey
Feb 20, 2018·Immunology and Cell Biology·Shweta TikooWolfgang Weninger
Jun 2, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takahito SaitoNaoko Nakano
Sep 19, 2017·Nature Reviews. Immunology·Morten M NielsenWendy L Havran
Oct 23, 2012·Cellular & Molecular Immunology·Shirin Kalyan, Dieter Kabelitz
Dec 21, 2012·European Journal of Immunology·Bruno Silva-SantosMatthias Eberl
Nov 27, 2018·Physiological Reviews·Melanie RodriguesGeoffrey C Gurtner
Sep 16, 2018·Immunology and Cell Biology·Katarzyna Mikolajewicz, Grzegorz Chodaczek
Jan 22, 2019·Nature Immunology·Benjamin E Willcox, Carrie R Willcox
Nov 24, 2012·Nature Reviews. Immunology·Philippe Bousso, Hélène D Moreau

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Methods Mentioned

BETA
confocal
transgenic
confocal microscopy
PMA
chemical modification
Fluorescence
flow cytometry

Software Mentioned

FlowJo
ImageJ
Slidebook
GraphPad
Leica Application Suite Advanced Fluorescence
GraphPad Prism

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