Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry

Scientific Reports
Jie MeiJulius Valentin Emmrich

Abstract

Body temperature is a valuable parameter in determining the wellbeing of laboratory animals. However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias. Here we compared two methods to assess body temperature in mice, namely implanted radio frequency identification (RFID) temperature transponders (method 1) to non-contact infrared thermometry (method 2) in 435 mice for up to 7 days during normothermia and lipopolysaccharide (LPS) endotoxin-induced hypothermia. There was excellent agreement between core and surface temperature as determined by method 1 and 2, respectively, whereas the intra- and inter-subject variation was higher for method 2. Nevertheless, using machine learning algorithms to determine temperature-based endpoints both methods had excellent accuracy in predicting death as an outcome event. Therefore, less expensive and cumbersome non-contact infrared thermometry can serve as a reliable alternative for implantable transponder-based systems for hypothermic responses, although requiring standardization between experimenters.

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Citations

Nov 22, 2019·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Philip PlaekeBenedicte Y De Winter
Nov 25, 2018·Biomedicines·Noof Abdullah ShaifInsop Shim
Aug 6, 2019·BioMed Research International·Noof Abdullah ShaifInsop Shim
Apr 3, 2020·Scientific Reports·Thi Cuc MaiRené de Seze
Jul 10, 2019·Computational Intelligence and Neuroscience·Lee Ben-Ami, Ido Bachelet
Feb 3, 2021·Physiology & Behavior·Catherine G FosterDavid W Howells
Mar 14, 2021·Physiology & Behavior·Wataru NishimuraYasuko Noda
Aug 12, 2021·Journal of Neurochemistry·Tatsuya ManabeMichael T Heneka
Sep 9, 2021·Advanced Science·Bozhidar-Adrian StefanovMartin Fussenegger

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Methods Mentioned

BETA
infrared thermometry

Software Mentioned

R Development Core Team
sklearn
Research Randomizer
rm
SPSS
R

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