PMID: 8947471Nov 15, 1996Paper

Bombesin and thrombin affect discrete pools of intracellular calcium through different G-proteins

The Biochemical Journal
J L WangN Gautam

Abstract

In mouse NIH 3T3 cells, the mitogens bombesin and thrombin induced Ca2+ release from intracellular stores. Ca2+ release induced by bombesin was inhibited by the Ca(2+)-ATPase inhibitor thapsigargin, while Ca2+ release induced by thrombin was unaffected by this agent. The Ca(2+)-release response to bombesin was not affected by pertussis toxin, but the response to thrombin was abolished by the toxin. Stable transfectants overexpressing the G-protein subunit type alpha 9 showed an accentuated response to bombesin, indicating that the bombesin receptor was coupled to a Gq-like G-protein. Together, these results show that the two mitogenic receptors are coupled to distinct G-proteins that affect functionally different pools of Ca2+. Organization of signalling pathways in this manner may allow cells to differentially encode information from different signals.

Citations

Sep 2, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·H WangW Zhang
Oct 23, 2002·Journal of Neurochemistry·Rosario GarridoMichael Mulholland
May 20, 1998·Journal of Molecular Neuroscience : MN·I V SmirnovaB W Festoff
Apr 25, 2000·Molecular and Cellular Biology·R L BoshansC D'Souza-Schorey
Mar 22, 2002·European Journal of Pharmacology·Sarah MasonShaun McNulty

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